CD30 Expression Identifies the Predominant Proliferating T Lymphocyte Population in Human Alloimmune Responses
| Autor: | Corwyn D. Hopke, Olivia M. Martinez, Sheri M. Krams, Keith W. Chan |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Isoantigens
CD30 T cell Immunology Population Antigen-Presenting Cells Ki-1 Antigen chemical and pharmacologic phenomena Stimulation Receptors Nerve Growth Factor In Vitro Techniques Biology Lymphocyte Activation Receptors Tumor Necrosis Factor Cell Line Flow cytometry Tumor Necrosis Factor Receptor Superfamily Member 9 Antigens CD T-Lymphocyte Subsets immune system diseases hemic and lymphatic diseases medicine Humans Immunology and Allergy IL-2 receptor education education.field_of_study integumentary system medicine.diagnostic_test CD137 Receptors Interleukin-2 hemic and immune systems T lymphocyte Receptors OX40 Molecular biology Tumor Necrosis Factor Receptor Superfamily Member 7 medicine.anatomical_structure Cytokines Leukocyte Common Antigens |
| Zdroj: | The Journal of Immunology. 169:1784-1791 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.169.4.1784 |
| Popis: | CD30 is an inducible member of the TNFR superfamily that is expressed on activated T and B cells and some lymphoid malignancies. We have previously shown that human CD30+ T cells elicited with allogeneic APC are a major source of IFN-γ and IL-5 production. In the present study we have used alloantigen, as well as anti-CD3 plus anti-CD28 mAb stimulation, to further characterize human CD30+ T cells with respect to function and the expression of other activation-dependent cell surface molecules, including the related TNFR family members OX-40 and 4-1BB (CD137). Our results indicate that human CD30+ T cells are a subset of activated T cells that also express CD25 and CD45RO. Moreover, we observed that allogeneic APC consistently induced a greater proportion of CD30+ cells within the activated T cell population than did stimulation with plate-bound anti-CD3 plus anti-CD28 mAb or stimulation with soluble anti-CD3 plus anti-CD28 and autologous APC. The enhanced induction of CD30 expression by alloantigen was not common to other inducible TNFR family members because anti-CD3 plus anti-CD28 mAbs were far more effective in inducing expression of 4-1BB and OX-40. Furthermore, CD30 expression marked the predominant proliferating T cell population induced by alloantigen as determined by CFSE staining and flow cytometry. These results indicate that CD30, but not 4-1BB or OX-40, is preferentially induced by alloantigen, suggesting that CD30 may be important in human alloimmune responses. |
| Databáze: | OpenAIRE |
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