Chitosan–Collagen Coated Magnetic Nanoparticles for Lipase Immobilization—New Type of 'Enzyme Friendly' Polymer Shell Crosslinking with Squaric Acid
| Autor: | Joanna Skopinska-Wisniewska, Adam Sikora, Halina Kaczmarek, Katarzyna Węgrzynowska-Drzymalska, Marta Ziegler-Borowska, Dorota Chełminiak-Dudkiewicz, Tomasz Siódmiak, Michał Piotr Marszałł |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
collagen
magnetic nanoparticles Immobilized enzyme Hydrochloride Nanoparticle 02 engineering and technology Squaric acid lcsh:Chemical technology 01 natural sciences Catalysis immobilization lipase chitosan squaric acid Chitosan lcsh:Chemistry chemistry.chemical_compound Polymer chemistry lcsh:TP1-1185 Physical and Theoretical Chemistry Lipase General Environmental Science biology 010405 organic chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry lcsh:QD1-999 biology.protein Magnetic nanoparticles Glutaraldehyde 0210 nano-technology |
| Zdroj: | Catalysts, Vol 7, Iss 1, p 26 (2017) Catalysts; Volume 7; Issue 1; Pages: 26 |
| ISSN: | 2073-4344 |
| Popis: | This article presents a novel route for crosslinking a polysaccharide and polysaccharide/protein shell coated on magnetic nanoparticles (MNPs) surface via condensation reaction with squaric acid (SqA). The syntheses of four new types of collagen-, chitosan-, and chitosan–collagen coated magnetic nanoparticles as supports for enzyme immobilization have been done. Structure and morphology of prepared new materials were characterized by attenuated total reflectance Fourier-transform infrared (ATR-FTIR), XRD, and TEM analysis. Next, the immobilization of lipase from Candida rugosa was performed on the nanoparticles surface via N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC)/N-hydroxy-succinimide (NHS) mechanism. The best results of lipase activity recovery and specific activities were observed for nanoparticles with polymer shell crosslinked via a novel procedure with squaric acid. The specific activity for lipase immobilized on materials crosslinked with SqA (52 U/mg lipase) was about 2-fold higher than for enzyme immobilized on MNPs with glutaraldehyde addition (26 U/mg lipase). Moreover, a little hyperactivation of lipase immobilized on nanoparticles with SqA was observed (104% and 112%). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|