The novel Arabidopsis thaliana svt2 suppressor of the ascorbic acid-deficient mutant vtc1-1 exhibits phenotypic and genotypic instability
| Autor: | Dulcie Lai, Karen C Vanderhoek, Meghan D Doerr, Aaron M. Khalid, Susan J. Lolle, Marianne Hopkins, Pei-Chun Chang |
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| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Plant Genomes & Evolution Evolutionary/Comparative Genetics 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Arabidopsis Genetic variation Genotype Arabidopsis thaliana Structure: RNA Arabidopsis genome instability genetic heterogeneity mosaicism inbreeding General Pharmacology Toxicology and Pharmaceutics 030304 developmental biology 2. Zero hunger Genetics 0303 health sciences Genetic diversity Plant Growth & Development General Immunology and Microbiology biology Mechanism (biology) Genetic heterogeneity food and beverages Articles Genomics General Medicine biology.organism_classification Developmental Evolution Evolutionary Ecology Plant Genetics & Gene Expression Structural Genomics Inbreeding Research Article Control of Gene Expression 010606 plant biology & botany |
| Zdroj: | F1000Research |
| ISSN: | 2046-1402 |
| DOI: | 10.12688/f1000research.2-5.v2 |
| Popis: | Concern over the tremendous loss of genetic diversity among many of our most important crops has prompted major efforts to preserve seed stocks derived from cultivated species and their wild relatives. Arabidopsis thaliana propagates mainly by self-fertilizing, and therefore, like many crop plants, theoretically has a limited potential for producing genetically diverse offspring. Despite this, inbreeding has persisted in Arabidopsis for over a million years suggesting that some underlying adaptive mechanism buffers the deleterious consequences of this reproductive strategy. Using presence-absence molecular markers we demonstrate that single Arabidopsis plants can have multiple genotypes. Sequence analyses reveal single nucleotide changes, loss of sequences and, surprisingly, acquisition of unique genomic insertions. Estimates based on quantitative analyses suggest that these genetically discordant sectors are very small but can have a complex genetic makeup. In ruling out more trivial explanations for these data, our findings raise the possibility that intrinsic drivers of genetic variation are responsible for the targeted sequence changes we detect. Given the evolutionary advantage afforded to populations with greater genetic diversity, we hypothesize that organisms that primarily self-fertilize or propagate clonally counteract the genetic cost of such reproductive strategies by leveraging a cryptic reserve of extra-genomic information. |
| Databáze: | OpenAIRE |
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