The quantitation of 2-oxo-3-hydroxy lysergic acid diethylamide (O-H-LSD) in human urine specimens, a metabolite of LSD: comparative analysis using liquid chromatography-selected ion monitoring mass spectrometry and liquid chromatography-ion trap mass spectrometry
| Autor: | Gregory K. Poch, Kevin L. Klette, Cynthia J. Anderson |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Detection limit
Chemical ionization Chemical Health and Safety Chromatography Health Toxicology and Mutagenesis Metabolite Reproducibility of Results Toxicology Mass spectrometry High-performance liquid chromatography Gas Chromatography-Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Lysergic Acid Diethylamide chemistry Evaluation Studies as Topic Environmental Chemistry Humans Selected ion monitoring Solid phase extraction Ion trap Chromatography High Pressure Liquid |
| Zdroj: | Journal of analytical toxicology. 24(3) |
| ISSN: | 0146-4760 |
| Popis: | This paper compares the potential forensic application of two sensitive and rapid procedures (liquid chromatography-mass spectrometry and liquid chromatography-ion trap mass spectrometry) for the detection and quantitation of 2-oxo-3-hydroxy lysergic acid diethylamide (O-H-LSD) a major LSD metabolite. O-H-LSD calibration curves for both procedures were linear over the concentration range 0-8,000 pg/mL with correlation coefficients (r2) greater than 0.99. The observed limit of detection (LOD) and limit of quantitation (LOQ) for O-H-LSD in both procedures was 400 pg/mL. Sixty-eight human urine specimens that had previously been found to contain LSD by gas chromatography-mass spectrometry were reanalyzed by both procedures for LSD and O-H-LSD. These specimens contained a mean concentration of O-H-LSD approximately 16 times higher than the LSD concentration. Because both LC methods produce similar results, either procedure can be readily adapted to O-H-LSD analysis for use in high-volume drug-testing laboratories. In addition, the possibility of significantly increasing the LSD detection time window by targeting this major LSD metabolite for analysis may influence other drug-free workplace programs to test for LSD. |
| Databáze: | OpenAIRE |
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