Evaluation of a High Throughput Starch Analysis Optimised for Wood
Autor: | Alessio Fini, Chandra Bellasio, Francesco Ferrini |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
0106 biological sciences
Starch lcsh:Medicine Carbohydrate Biosynthesis Plant Science 01 natural sciences Biochemistry Plant Roots Trees Analytical Chemistry Matrix (chemical analysis) chemistry.chemical_compound Food science lcsh:Science chemistry.chemical_classification 0303 health sciences Multidisciplinary Ecology Plant Biochemistry Hydrolysis food and beverages Plants Hydrogen-Ion Concentration Wood Chemistry Shoot Plant Shoots Research Article Flowers Polysaccharide 03 medical and health sciences Polysaccharides Plant-Environment Interactions Botany Cellulose Biology 030304 developmental biology Plant Ecology lcsh:R Reproducibility of Results Starch analysis Rhizome Plant Leaves Glucose chemistry lcsh:Q Plant Biotechnology glucose 010606 plant biology & botany |
Zdroj: | PLoS ONE PloS one 9 (2014): Article Number: e86645. doi:10.1371/journal.pone.0086645 info:cnr-pdr/source/autori:Bellasio C.; Fini A.; Ferrini F./titolo:Evaluation of a high throughput starch analysis optimised for wood/doi:10.1371%2Fjournal.pone.0086645/rivista:PloS one/anno:2014/pagina_da:Article Number: e86645/pagina_a:/intervallo_pagine:Article Number: e86645/volume:9 PLoS ONE, Vol 9, Iss 2, p e86645 (2014) |
ISSN: | 1932-6203 |
Popis: | Starch is the most important long-term reserve in trees, and the analysis of starch is therefore useful source of physiological information. Currently published protocols for wood starch analysis impose several limitations, such as long procedures and a neutralization step. The high-throughput standard protocols for starch analysis in food and feed represent a valuable alternative. However, they have not been optimised or tested with woody samples. These have particular chemical and structural characteristics, including the presence of interfering secondary metabolites, low reactivity of starch, and low starch content. In this study, a standard method for starch analysis used for food and feed (AOAC standard method 996.11) was optimised to improve precision and accuracy for the analysis of starch in wood. Key modifications were introduced in the digestion conditions and in the glucose assay. The optimised protocol was then evaluated through 430 starch analyses of standards at known starch content, matrix polysaccharides, and wood collected from three organs (roots, twigs, mature wood) of four species (coniferous and flowering plants). The optimised protocol proved to be remarkably precise and accurate (3%), suitable for a high throughput routine analysis (35 samples a day) of specimens with a starch content between 40 mg and 21 mg. Samples may include lignified organs of coniferous and flowering plants and non-lignified organs, such as leaves, fruits and rhizomes. © 2014 Bellasio et al. |
Databáze: | OpenAIRE |
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