QTL mapping of root traits in wheat under different phosphorus levels using hydroponic culture
| Autor: | Xianchun Xia, Yang Mengjiao, Muhammad Hassan, Shu-bing Shi, Zhonghu He, Faji Li, Yonggui Xiao, Wang Cairong |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Quantitative trait loci lcsh:QH426-470 lcsh:Biotechnology Population Root system Biology Quantitative trait locus 01 natural sciences 03 medical and health sciences Hydroponics lcsh:TP248.13-248.65 Inclusive composite interval mapping Genetics Grain quality Allele education Triticum 030304 developmental biology 0303 health sciences education.field_of_study Chromosome Mapping Phosphorus Root traits biology.organism_classification lcsh:Genetics Plant Breeding Horticulture Phenotype Genome-wide linkage mapping Seedling Doubled haploidy SNP array Research Article 010606 plant biology & botany Biotechnology |
| Zdroj: | BMC Genomics BMC Genomics, Vol 22, Iss 1, Pp 1-12 (2021) |
| Popis: | Background Phosphorus (P) is an important in ensuring plant morphogenesis and grain quality, therefore an efficient root system is crucial for P-uptake. Identification of useful loci for root morphological and P uptake related traits at seedling stage is important for wheat breeding. The aims of this study were to evaluate phenotypic diversity of Yangmai 16/Zhongmai 895 derived doubled haploid (DH) population for root system architecture (RSA) and biomass related traits (BRT) in different P treatments at seedling stage using hydroponic culture, and to identify QTL using 660 K SNP array based high-density genetic map. Results All traits showed significant variations among the DH lines with high heritabilities (0.76 to 0.91) and high correlations (r = 0.59 to 0.98) among all traits. Inclusive composite interval mapping (ICIM) identified 34 QTL with 4.64–20.41% of the phenotypic variances individually, and the log of odds (LOD) values ranging from 2.59 to 10.43. Seven QTL clusters (C1 to C7) were mapped on chromosomes 3DL, 4BS, 4DS, 6BL, 7AS, 7AL and 7BL, cluster C5 on chromosome 7AS (AX-109955164 - AX-109445593) with pleiotropic effect played key role in modulating root length (RL), root tips number (RTN) and root surface area (ROSA) under low P condition, with the favorable allele from Zhongmai 895. Conclusions This study carried out an imaging pipeline-based rapid phenotyping of RSA and BRT traits in hydroponic culture. It is an efficient approach for screening of large populations under different nutrient conditions. Four QTL on chromosomes 6BL (2) and 7AL (2) identified in low P treatment showed positive additive effects contributed by Zhongmai 895, indicating that Zhongmai 895 could be used as parent for P-deficient breeding. The most stable QTL QRRS.caas-4DS for ratio of root to shoot dry weight (RRS) harbored the stable genetic region with high phenotypic effect, and QTL clusters on 7A might be used for speedy selection of genotypes for P-uptake. SNPs closely linked to QTLs and clusters could be used to improve nutrient-use efficiency. |
| Databáze: | OpenAIRE |
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