Expression scanning of an array of growth control genes in human tumor cell lines
| Autor: | Samuel Granjeaud, S Van Hulst, Karine Bernard, Bertrand Jordan, Béatrice Loriod, Daniel Birnbaum, M Starkey, Rebecca Tagett, François Bertucci, Catherine Nguyen |
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| Přispěvatelé: | Technologies avancées pour le génôme et la clinique (TAGC), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
DNA Complementary [SDV]Life Sciences [q-bio] Breast Neoplasms Computational biology Biology medicine.disease_cause 03 medical and health sciences Nucleic acid thermodynamics 0302 clinical medicine Neoplasms Gene expression Genetics medicine Image Processing Computer-Assisted Tumor Cells Cultured Humans Molecular Biology Gene ComputingMilieux_MISCELLANEOUS 030304 developmental biology Regulation of gene expression 0303 health sciences Cell growth Immunity Nucleic Acid Hybridization Reproducibility of Results Membranes Artificial Gene Expression Regulation Neoplastic 030220 oncology & carcinogenesis Subtraction Technique Female DNA microarray Transcription Factor Gene Carcinogenesis Cell Division |
| Zdroj: | Oncogene Oncogene, 1999, 18 (26), pp.3905-3912. ⟨10.1038/sj.onc.1202731⟩ Oncogene, Nature Publishing Group, 1999, 18 (26), pp.3905-3912. ⟨10.1038/sj.onc.1202731⟩ |
| ISSN: | 0950-9232 1476-5594 |
| Popis: | Analysis of gene expression on a medium- or large-scale is an increasingly recognized method for functional and clinical investigations based on the now extensive catalog of known or partially sequenced genes. The accessibility of this approach can be enhanced by using readily available technology (macroarrays on Nylon, radioactive detection) and the IMAGE resource to assemble sets of targets. We have set up such a medium-scale, flexible system and validated it by the study of quantitative expression levels for 120 genes in six cell lines, including three mammary carcinoma cell lines. A number of important parameters are identified as necessary for the assembly of a valid set and the obtention of good-quality quantitative data. The extensive data assembled in this survey identified potential targets of carcinogenesis, for example the CRABP2 and GATA3 transcription factor genes. We also demonstrate the feasibility of this procedure for relatively small tumor samples, without recourse to probe amplification methods. |
| Databáze: | OpenAIRE |
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