High LINC00536 expression promotes tumor progression and poor prognosis in bladder cancer
| Autor: | Chen Zhu, Long Ma, Lei Zhang, Ran Li, Zhonglei Deng, Jingyuan Tang, Zhiqiang Qin, Yunfei Wei |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell Mice Nude Biology Cell Line 03 medical and health sciences Mice 0302 clinical medicine In vivo Cell Line Tumor Wnt3A Protein medicine Biomarkers Tumor Animals Humans Wnt Signaling Pathway beta Catenin Cell Proliferation Gene knockdown Bladder cancer Cancer Cell Biology medicine.disease 030104 developmental biology medicine.anatomical_structure Urinary Bladder Neoplasms Cell culture Tumor progression 030220 oncology & carcinogenesis Cancer research Female RNA Long Noncoding WNT3A |
| Zdroj: | Experimental cell research. 378(1) |
| ISSN: | 1090-2422 |
| Popis: | Growing evidences demonstrate that long non-coding RNAs (lncRNAs) contribute to the cancer initiation and progression and are considered as promising diagnostic and therapeutic targets of multiple cancers. However, the definite role of LINC00536 in bladder cancer (BC) remains unclear. In the present study, we found LINC00536 expression was highly expressed in BC tissues compared with controls and negatively associated with survival rate in BC patients. Gain-of-function assays indicated that LINC00536 overexpression promoted the proliferation, migration and invasion, whereas LINC00536 knockdown attenuated the cell phenotypes above in BC cell lines. In vivo assay illustrated that LINC00536 knockdown inhibited BC growth in vivo. Mechanistically, Wnt3a was identified as the target of LINC00536. LINC00536 promoted malignant phenotypes via activating the Wnt3a/β-Catenin signaling. Wnt3a knockdown reversed the increase of proliferation, migration, and invasion abilities of BC cells induced by LINC00536 overexpression. In summary, our findings demonstrated that LINC00536 promoted BC progression by modulating the Wnt3a/β-Catenin signaling. |
| Databáze: | OpenAIRE |
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