Severe magnesium deficiency compromises systemic bone mineral density and aggravates inflammatory bone resorption
| Autor: | Carlos Rossa, Gabriela Giro, Rafael Scaf de Molon, Marina Montosa Belluci, S. R. P. Orrico, Paulo Sérgio Cerri, Elcio Marcantonio, Sotirios Tetradis |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), UCLA School of Dentistry, UCLA, Guarulhos University |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Deoxypyridinoline Endocrinology Diabetes and Metabolism Clinical Biochemistry Osteoporosis Osteoclasts Parathyroid hormone Core Binding Factor Alpha 1 Subunit Bone resorption Biochemistry Bone remodeling chemistry.chemical_compound 0302 clinical medicine Bone Density Medicine Magnesium Osteopontin Bone mineral Nutrition and Dietetics biology Parathyroid Hormone Periodontal disease musculoskeletal diseases medicine.medical_specialty Osteocalcin 03 medical and health sciences Osteoprotegerin Internal medicine Animals RNA Messenger Bone Resorption Periodontitis Molecular Biology Inflammation Interleukin-6 business.industry X-Ray Microtomography 030206 dentistry Alkaline Phosphatase medicine.disease Magnesium deficiency Rats 030104 developmental biology Endocrinology chemistry X-ray microtomography biology.protein Calcium business Magnesium Deficiency Biomarkers |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2020-12-12T01:48:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) We sought to evaluate the effects of magnesium (Mg) intake deficiency on bone metabolism in rats with induced periodontal disease (PD). Holtzman rats were randomly divided into two groups: Control — animals fed a standard diet and test — animals fed a diet with 90% Mg deficiency. After 60 days on the diets, all animals received ligature on the lower left first molars to induce PD. Animals were euthanized after 30 days following ligature placement. Blood and urine were collected for determination of serum concentrations of Mg, calcium, osteocalcin (OCN), alkaline phosphatase and parathyroid hormone (PTH) by enzyme-linked immunosorbent assay, and the urinary concentration of deoxypyridinoline (DPD). Systemic bone mineral density (BMD), bone volume and architectural bone parameters were evaluated by micro-CT in L4 lumbar vertebrae and mandible. Tartrate-resistant acid phosphatase staining and immunohistochemical (IHC) analysis of inducible nitric oxide synthase (iNOS), Runt-related transcription factor 2 (RUNX2), CD86, CD80, proliferating cell nuclear antigen, vascular endothelial growth factor, OCN and osteopontin were investigated. Reverse-transcription polymerase chain reaction was employed to assess mRNA expression of receptor-activator of nuclear factor-kB ligand, osteoprotegerin (OPG) and interleukin (IL)-6. Mg deficiency was associated with higher concentrations of PTH and DPD, and significant decrease on both systemic and mandibular BMD, as well as greater severity of alveolar and trabecular bone loss. Significant increase in osteoclasts was observed in the test group with PD. IHC analysis showed significant increase in the expression of iNOS and decreased expression of OCN and RUNX2. Increased IL-6 mRNA and decreased OPG mRNA expressions were evidenced in the test group with PD. Mg deficiency caused systemic effects indicative of altered bone metabolism in the vertebrae and affected both immune and stromal cells, aggravating inflammatory bone resorption in the ligature-induced model of periodontitis. Department of Diagnosis and Surgery Sao Paulo State University School of Dentistry at Araraquara–UNESP Division of Diagnostic and Surgical Sciences UCLA School of Dentistry Molecular Biology Institute UCLA Department of Periodontology and Oral Implantology Dental Research Division Guarulhos University PhD Department of Morphology Sao Paulo State University School of Dentistry at Araraquara–UNESP Department of Diagnosis and Surgery Sao Paulo State University School of Dentistry at Araraquara–UNESP PhD Department of Morphology Sao Paulo State University School of Dentistry at Araraquara–UNESP FAPESP: 2006/05774-0 FAPESP: 2008/04855-2 FAPESP: 2015/21697-5 CAPES: AUXPE 655/2014 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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