In Vitro Anticancer Activity of Imperata cylindrica Root’s Extract toward Human Cervical Cancer and Identification of Potential Bioactive Compounds
| Autor: | Armelle T. Mbaveng, Victor Kuete, Paul Nayim, Krishna Sudhir, Mukherjee Sanjukta |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Jatrorrhizine
Cell cycle checkpoint Article Subject General Immunology and Microbiology medicine.diagnostic_test biology General Medicine Cell cycle Pharmacology biology.organism_classification General Biochemistry Genetics and Molecular Biology Flow cytometry HeLa chemistry.chemical_compound chemistry Apoptosis medicine Caffeic acid Medicine Myricetin Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2021 (2021) |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2021/4259777 |
| Popis: | Imperata cylindrica is traditionally used to cure several diseases including cancer, wounds, and hypertension. The present study was designed to investigate the anticancer activity of the methanolic root extract of I. cylindrica (IC-MeOH). The water-soluble tetrazolium-1 and colony formation assays were used to check the proliferation ability of the cells. Cell apoptosis and cell cycle were measured by flow cytometry-based fluorescence-activated cell sorting. The ultrahigh-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) analysis was used for the metabolites profiling of IC-MeOH. Based on high-mass accuracy, spectral data, and previous reports, tentative compound identifications were assigned. Our findings revealed that IC-MeOH inhibited the proliferation of HeLa and CaSki cells. The plant extract was also found to induce a concentration- and time-dependent apoptosis and cell cycle arrest in the G0/G1 phase (IC50 value) in CaSki cell line. Analysis of IC-MeOH permitted the identification of 10 compounds already reported for their anticancer activity, epicatechin, curcumin, (-)-yatein, caffeic acid, myricetin, jatrorrhizine, harmaline, cinnamaldehyde, dobutamine, and syringin. In conclusion, IC-MeOH is a rich source of cytotoxic metabolites that inhibits human cervical cancer proliferation via apoptosis and cell cycle arrest. |
| Databáze: | OpenAIRE |
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