Intestinal cestodes of chicken are effectively killed by quinoline-rich extract of Spilanthes acmella
| Autor: | Pawi Bawitlung Lalthanpuii, Kholhring Lalchhandama |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Antiparasitic
medicine.drug_class Veterinary medicine helminthiasis Acmella Spilanthes acmella SF1-1100 030308 mycology & parasitology Albendazole 03 medical and health sciences quinoline SF600-1100 medicine Helminths Anthelmintic cestode 030304 developmental biology 0303 health sciences General Veterinary Traditional medicine biology Chemistry Viral tegument biology.organism_classification Animal culture Raillietina tetragona tegument Rostellum (helminth) medicine.drug Research Article |
| Zdroj: | Veterinary World Veterinary World, Vol 13, Iss 4, Pp 821-826 (2020) |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | Background and Aim: Spilanthes acmella is used for the treatment of intestinal helminth infections in Mizo traditional medicine. In spite of a variety of drugs developed for helminthiases, an entirely safe and absolutely effective drug is still lacking, so much so that infections remain a major problem in human and animal welfare. In this study, we attempted to substantiate S. acmella as an anticestodal agent. Materials and Methods: The aqueous extract of the aerial parts of S. acmella was prepared and from it a bioactive fraction was obtained using column chromatography. Chemical analyses were done using thin-layer chromatography (TLC) and gas chromatography–mass spectrometry (GC–MS). Helminth survival test was performed in vitro on an intestinal cestode, Raillietina tetragona. Structural effects on the cestode were examined under scanning electron microscopy. Results: From the bioactive fraction of S. acmella extract, TLC indicated the presence of an aromatic quinone, which was identified using GC–MS as a quinoline derivative (2,2,4-trimethyl-1,2-dihydroquinoline having a retention time of 24.97 min and chemical formula of C12H15N). The quinoline-rich fraction showed concentration-dependent activity against R. tetragona as that of albendazole. Scanning electron microscopy of the treated cestode revealed classic anthelmintic effects such as tegumental shrinkage and damage of surface organs. The scolex was shrunk, suckers were degenerated with disintegrated spines, and rostellum was completely collapsed. There were severe damages on the tegument and formation of pit-like scars on the proglottids. Conclusion: The efficacy of S. acmella extract and structural damages it caused on the cestode indicates that it is a potential source of anthelmintic agent and that 2,2,4-trimethyl-1,2-dihydroquinoline contributes to its antiparasitic activity. |
| Databáze: | OpenAIRE |
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