GLUTAMINE DEPRIVATION EFFECT ON DEK, TPD52, BRCA1, ADGRE5, LIF, GNPDA1, AND COL6A1 GENE EXPRESSIONS IN IRE1 KNOCKDOWN U87 GLIOMA CELLS
| Autor: | O. Y. Luzina, Y. A. Garmash, A. P. Kharkova, A.Y. Kuznetsova, O. H. Minchenko, S. V. Danilovskyi, O. S. Hnatiuk |
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| Rok vydání: | 2017 |
| Předmět: |
U87 glioma cells
0301 basic medicine IRE1 knockdown ADGRE5 lcsh:Biotechnology Mrna expression COL6A1 GNPDA1 genes 03 medical and health sciences lcsh:TP248.13-248.65 Glioma medicine glutamine deprivation U87 neoplasms Gene Gene knockdown Chemistry DEK mRNA expression BRCA1 medicine.disease nervous system diseases Cell biology Glutamine 030104 developmental biology |
| Zdroj: | Biotechnologia Acta, Vol 10, Iss 6, Pp 18-27 (2017) |
| ISSN: | 2410-776X 2410-7751 |
| DOI: | 10.15407/biotech10.06.018 |
| Popis: | To study effect of glutamine deprivation on the expression of genes encoding the key proliferation associated factors on a relation to inhibition of inositol requiring enzyme-1 IRE1 in U87 glioma cells was the aim of the research. It was shown that glutamine withdrawal down-regulated the expression of DEK, BRCA1, LIF, and COL6A1 genes in control glioma cells (transfected by empty vector), up-regulated ADGRE5 gene expression, and did not significantly change the expression of TPD52 and GNPDA. Inhibition of ІRE1 signaling enzyme activity modified the effect of glutamine deprivation on the expression of TPD52, BRCA1, LIF, DEK, ADGRE5, and COL6A1 genes: introduces the effect of glutamine deprivation on TPD52 and GNPDA1, reduced – on COL6A1, and enhanced – on ADGRE5, DEK, and BRCA1 in U87 glioma cells. Therefore, glutamine deprivation affect the expression level of most studied genes in U87 glioma cells in relation to the functional activity of IRE1 signaling enzyme, which is responsible for control of cell proliferation and glioma growth. |
| Databáze: | OpenAIRE |
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