Chemical fingerprinting of three Anemone species and an adulteration study to detect cross mixing of medicinal plants by HPLC-HR-ESI-MS/MS method
| Autor: | Hamna Shadab, Muhammad Noman Khan, Syed Ghulam Musharraf, Adeeba Khadim, Muzna Syed, Hesham R. El-Seedi, Anjum Perveen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Electrospray
Science (General) Electrospray ionization Anemone obtusiloba 02 engineering and technology 010501 environmental sciences Ziziphus jujube 01 natural sciences High-performance liquid chromatography Analytical Chemistry Q1-390 food Analytisk kemi LC-ESI-MS Medicinal plants 0105 earth and related environmental sciences Multidisciplinary Chromatography biology Chemistry Botany food and beverages Anemone MS Botanik LC-ESI-MS/MS 021001 nanoscience & nanotechnology biology.organism_classification food.food Anemone falconeri Ziziphus jujuba Plant species Anemone tetrasepala 0210 nano-technology Chemical fingerprinting |
| Zdroj: | Journal of King Saud University: Science, Vol 33, Iss 5, Pp 101461-(2021) |
| Popis: | The adulteration of plant raw materials used for the preparation of herbal drugs with foreign plant material is one of the important issues in the quality control of herbal products. Chemical fingerprinting is a well-known approach for the characterization of secondary metabolites associated with the plant species and can be used for quality control of plant material. The current study centred on the development of chemical fingerprinting of three medicinal plants of genus Anemone including A. obtusiloba, A. falconeri and A tetrasepala through identification of their metabolites using LC-ESI-QTOF-MS/MS analysis. Thirty compounds were identified by using high-resolution positive and negative electrospray- ionization (ESI) modes and MS/MS analysis. The identified compounds belong to diterpenoids, alkaloids, phenols, flavonoids and other classes and their distribution among the analysed species was studied using different statistical tools. Moreover, an LC-HR-ESI-MS/MS method was developed to detect the cross mixing of A. obtusiloba with Ziziphus jujuba. Seven chromatographically differentiative peaks confined to A. obtusiloba were selected to detect its contamination in adulterated samples. The method was able to detect as low as 20% mixing of A. obtusiloba in Z. jujuba. This study can play a significant role to manage the quality control of herbal medicines and to identify lead natural products of these plants. |
| Databáze: | OpenAIRE |
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