Molecular subtypification of human papillomavirus in male adult individuals with recurrent respiratory papillomatosis
| Autor: | Patricia Canto, Ana Karla Guzmán-Romero, Matsuharu Akaki-Caballero, Carmen S. García-Romero, Ramón Mauricio Coral-Vázquez, Ana Graciela M Saavedra-Mendoza |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male medicine.medical_specialty Genotype Vocal Cords Disease Respiratory papillomatosis Polymerase Chain Reaction Linear array Young Adult Viral type Internal medicine medicine Humans Human papillomavirus Mexico Respiratory Tract Infections Aged Human papillomavirus 16 Human papillomavirus 11 business.industry Papillomavirus Infections General Medicine Middle Aged Human papillomavirus 6 Isotype Otorhinolaryngology DNA Viral Immunology Etiology Surgery Recurrent Respiratory Papillomatosis business |
| Zdroj: | Auris Nasus Larynx. 42:385-389 |
| ISSN: | 0385-8146 |
| DOI: | 10.1016/j.anl.2015.03.006 |
| Popis: | Objective This study aimed to identify the isotype of human papillomavirus (HPV) in fresh tissue samples of 35 male adults with adult recurrent adult respiratory papillomatosis which may be important to define the precise etiology of the disease, and determine the therapeutic and prophylactic measures. Methods A total of 35 adult male patients diagnosed with active RRP who have been treated for several years were included in the study. DNA of patients was extracted from fresh biological samples and analyzed by PCR and a Linear Array ® HPV Genotyping system. Results Most cases (95%) corresponded to adult-onset of RRP. A questionnaire was applied to obtain demographic and clinical data. Using a PCR-based detection system all patients showed the presence of HPV; 80% were positive for HPV-6, 8% for HPV-11 and one for HPV-16. Conclusion Most patients presented HPV-6 and consequently it was not feasible to correlate clinical and demographic characteristics with viral type. Besides, co-infections were not evident. This knowledge may be relevant to delineate therapeutic and preventive measures. |
| Databáze: | OpenAIRE |
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