HSM2 (HMO1) gene participates in mutagenesis control in yeast Saccharomyces cerevisiae
| Autor: | Irina V. Fedorova, S. V. Kovaltsova, V. T. Peshekhonov, T.A. Evstukhina, S.Yu. Alekseev, L. M. Gracheva, Vladimir G. Korolev |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Saccharomyces cerevisiae Proteins
DNA Repair DNA repair Base Pair Mismatch Cell Survival Ultraviolet Rays Mutant Saccharomyces cerevisiae Genes Fungal Mutagenesis (molecular biology technique) DNA-Directed DNA Polymerase medicine.disease_cause Biochemistry Polymerase Chain Reaction Gene product Fungal Proteins medicine Mutation frequency DNA Fungal Molecular Biology DNA Primers Genetics Mutation Endodeoxyribonucleases biology High Mobility Group Proteins Cell Biology biology.organism_classification Molecular biology Neoplasm Proteins DNA-Binding Proteins MutL Proteins Mutagenesis DNA mismatch repair Carrier Proteins DNA Damage Molecular Chaperones |
| Zdroj: | DNA repair. 1(4) |
| ISSN: | 1568-7864 |
| Popis: | We have previously reported about a new Saccharomyces cerevisiae mutation, hsm2-1, that results in increase of both spontaneous and UV-induced mutation frequencies but does not alter UV-sensitivity. Now HSM2 gene has been genetically and physically mapped and identified as a gene previously characterized as HMO1, a yeast homologue of human high mobility group genes HMG1/2. We found that hsm2 mutant is slightly deficient in plasmid-borne mismatch repair. We tested UV-induced mutagenesis in double mutants carrying hsm2-1 mutation and a mutation in a gene of principal damaged DNA repair pathways (rad2 and rev3) or in a mismatch repair gene (pms1 and recently characterized in our laboratory hsm3). The frequency of UV-induced mutations in hsm2 rev3 was not altered in comparison with single rev3 mutant. In contrast, the interaction of hsm2-1 with rad2 and pms1 was characterized by an increased frequency of UV-induced mutations in comparison with single rad2 and pms1 mutants. The UV-induced mutation frequency in double hsm2 hsm3 mutant was lower than in the single hsm2 and hsm3 mutants. The role of the HSM2 gene product in control of mutagenesis is discussed. |
| Databáze: | OpenAIRE |
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