Solanaceous exocyst subunits are involved in immunity to diverse plant pathogens

Autor: Elysa J. R. Overdijk, Klaas Bouwmeester, Francine Govers, Jeroen A. Berg, Yu Du
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Multiprotein complex
Phytophthora infestans
Physiology
Pseudomonas syringae
Nicotiana benthamiana
Exocyst
Plant Science
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
gene silencing
Laboratorium voor Plantenveredeling
Solanum lycopersicum
Arabidopsis
Tobacco
Plant Immunity
Secretion
Plant Diseases
Solanum tuberosum
biology
phylogenetic analysis
Callose
fungi
Basal defence
food and beverages
Laboratorium voor Celbiologie
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Solanaceous plants
Research Papers
plant resistance
Cell biology
Laboratorium voor Phytopathologie
Plant Breeding
Laboratory of Cell Biology
030104 developmental biology
chemistry
Plant—Environment Interactions
Laboratory of Phytopathology
vesicle trafficking
Botrytis
exocyst complex
EPS
exocytosis
010606 plant biology & botany
Zdroj: Journal of Experimental Botany, 69(3), 655-666
Journal of Experimental Botany 69 (2018) 3
Journal of Experimental Botany
ISSN: 0022-0957
DOI: 10.1093/jxb/erx442
Popis: This study provides insight into the evolution of the exocyst subunits of Solanaceous plants and is the first to show their role in immunity against multiple unrelated pathogens.
The exocyst, a multiprotein complex consisting of eight subunits, plays an essential role in many biological processes by mediating secretion of post-Golgi-derived vesicles towards the plasma membrane. In recent years, roles for plant exocyst subunits in pathogen defence have been uncovered, largely based on studies in the model plant Arabidopsis. Only a few studies have been undertaken to assign the role of exocyst subunits in plant defence in other plants species, including crops. In this study, predicted protein sequences from exocyst subunits were retrieved by mining databases from the Solanaceous plants Nicotiana benthamiana, tomato, and potato. Subsequently, their evolutionary relationship with Arabidopsis exocyst subunits was analysed. Gene silencing in N. benthamiana showed that several exocyst subunits are required for proper plant defence against the (hemi-)biotrophic plant pathogens Phytophthora infestans and Pseudomonas syringae. In contrast, some exocyst subunits seem to act as susceptibility factors for the necrotrophic pathogen Botrytis cinerea. Furthermore, the majority of the exocyst subunits were found to be involved in callose deposition, suggesting that they play a role in basal plant defence. This study provides insight into the evolution of exocyst subunits in Solanaceous plants and is the first to show their role in immunity against multiple unrelated pathogens.
Databáze: OpenAIRE
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