Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate
Autor: | Shen Peijie, Rongfei Hu, Lina Qin, Xianzhang Jiang, Ting Xue, Jianzhong Huang, Feng Qi, Youqiang Chen |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
Torulene lcsh:Biotechnology Mutant Rhodosporidium toruloides Management Monitoring Policy and Law 01 natural sciences Applied Microbiology and Biotechnology lcsh:Fuel Hydrolysate 03 medical and health sciences chemistry.chemical_compound Hydrolysis lcsh:TP315-360 lcsh:TP248.13-248.65 010608 biotechnology Tea waste hydrolysate Aromatic amino acids Food science Carotenoid 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Renewable Energy Sustainability and the Environment R. toruloides food and beverages Metabolism Lipid biology.organism_classification General Energy chemistry Torularhodin Biotechnology |
Zdroj: | Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-12 (2020) |
ISSN: | 1754-6834 |
DOI: | 10.1186/s13068-020-01712-0 |
Popis: | Background In this study, renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production. A novel Rhodosporidium toruloides mutant strain, RM18, was isolated through atmospheric and room-temperature plasma mutagenesis and continuous domestication in tea waste hydrolysate from R. toruloides ACCC20341. Results RM18 produced a larger biomass and more carotenoids and α-linolenic acid compared with the control strain cultured in tea waste hydrolysate. The highest yields of torularhodin (481.92 μg/g DCW) and torulene (501 μg/g DCW) from RM18 cultured in tea waste hydrolysate were 12.86- and 1.5-fold higher, respectively, than that of the control strain. In addition, α-linolenic acid production from RM18 in TWH accounted for 5.5% of total lipids, which was 1.58 times more than that of the control strain. Transcriptomic profiling indicated that enhanced central metabolism and terpene biosynthesis led to improved carotenoids production, whereas aromatic amino acid synthesis and DNA damage checkpoint and sensing were probably relevant to tea waste hydrolysate tolerance. Conclusion Tea waste is suitable for the hydrolysis of microbial cell culture mediums. The R. toruloides mutant RM18 showed considerable carotenoids and lipid production cultured in tea waste hydrolysate, which makes it viable for industrial applications. |
Databáze: | OpenAIRE |
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