Export of vacuolar manganese by AtNRAMP3 and AtNRAMP4 is required for optimal photosynthesis and growth under manganese deficiency
| Autor: | Françoise Lelièvre, Magali Schnell Ramos, Hélène Barbier-Brygoo, Viviane Lanquar, Ute Krämer, Sébastien Thomine, Anja Krieger-Liszkay |
|---|---|
| Přispěvatelé: | Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Service de Bioénergétique, Biologie Stucturale, et Mécanismes (SB2SM), Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie et de Technologies de Saclay (IBITECS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, Institut des sciences du végétal ( ISV ), Centre National de la Recherche Scientifique ( CNRS ), Service de Bioénergétique, Biologie Stucturale, et Mécanismes ( SB2SM ), Centre National de la Recherche Scientifique ( CNRS ) -Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ), Institut de Biologie et de Technologies de Saclay ( IBITECS ), Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ), Max Planck Institute of Molecular Plant Physiology, Max-Planck-Institut, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
[ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology Photosystem II Physiology MESH: Biological Transport MESH: Manganese Plant Science Vacuole MESH: Arabidopsis Proteins Manganese deficiency (plant) Photosynthesis 01 natural sciences MESH : Cation Transport Proteins MESH : Arabidopsis Proteins MESH: Vacuoles Superoxide dismutase MESH : Protoplasts 03 medical and health sciences MESH : Plant Leaves MESH: Cation Transport Proteins MESH : Arabidopsis Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology MESH: Arabidopsis MESH : Superoxide Dismutase MESH: Photosynthesis MESH: Superoxide Dismutase 030304 developmental biology 0303 health sciences biology Wild type food and beverages MESH: Protoplasts MESH : Photosynthesis Chloroplast MESH: Plant Leaves MESH : Biological Transport Biochemistry biology.protein MESH : Manganese Plant nutrition MESH : Vacuoles 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2010, 152 (4), pp.1986-99. ⟨10.1104/pp.109.150946⟩ Plant Physiology, American Society of Plant Biologists, 2010, 152 (4), pp.1986-99. 〈10.1104/pp.109.150946〉 Plant Physiology, 2010, 152 (4), pp.1986-99. ⟨10.1104/pp.109.150946⟩ |
| ISSN: | 0032-0889 1532-2548 |
| DOI: | 10.1104/pp.109.150946⟩ |
| Popis: | International audience; Manganese (Mn) is an essential element, acting as cofactor in numerous enzymes. In particular, a Mn cluster is indispensable for the function of the oxygen-evolving complex of photosystem II. Metal transporters of the Natural Resistance-Associated Macrophage Protein (NRAMP) family have the ability to transport both iron and Mn. AtNRAMP3 and AtNRAMP4 are required for iron mobilization in germinating seeds. The results reported here show that, in adult Arabidopsis (Arabidopsis thaliana) plants, AtNRAMP3 and AtNRAMP4 have an important role in Mn homeostasis. Vacuolar Mn accumulation in mesophyll cells of rosette leaves of adult nramp3nramp4 double mutant plants was dramatically increased when compared with the wild type. This suggests that a considerable proportion of the cellular Mn pool passes through the vacuole and is retrieved in an AtNRAMP3/AtNRAMP4-dependent manner. The impaired Mn release from mesophyll vacuoles of nramp3nramp4 double mutant plants is associated with reduced growth under Mn deficiency. However, leaf AtNRAMP3 and AtNRAMP4 protein levels are unaffected by Mn supply. Under Mn deficiency, nramp3nramp4 plants contain less functional photosystem II than the wild type. These data are consistent with a shortage of Mn to produce functional photosystem II, whereas mitochondrial Mn-dependent superoxide dismutase activity is maintained under Mn deficiency in both genotypes. The results presented here suggest an important role for AtNRAMP3/AtNRAMP4-dependent Mn transit through the vacuole prior to the import into chloroplasts of mesophyll cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|