Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate x Rosa rugosa waste
| Autor: | Mengqi Wu, Xu Zhizhen, Wenqing Zhang, Yilin Zhang, Wei Li, Wei Xia, Lei Shi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Arabinose
Polymers and Plastics Rhamnose Size-exclusion chromatography 02 engineering and technology Rosa 010402 general chemistry Polysaccharide 01 natural sciences Mice chemistry.chemical_compound Phagocytosis Materials Chemistry Animals Hypoglycemic Agents Immunologic Factors Bioassay Glycoside Hydrolase Inhibitors chemistry.chemical_classification biology Plant Extracts Hexuronic Acids Macrophages Organic Chemistry Rugosa Galactose alpha-Glucosidases 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Congo red Molecular Weight RAW 264.7 Cells chemistry Biochemistry Cytokines Pectins alpha-Amylases 0210 nano-technology Signal Transduction |
| Zdroj: | Carbohydrate Polymers. 253:117190 |
| ISSN: | 0144-8617 |
| Popis: | In this study, two polysaccharide components named WSRP-2a and WSRP-2b, were purified from Rosa setate x Rosa rugosa waste via anion exchange and gel filtration chromatography. Monosaccharide composition, Congo red assay, FT-IR and NMR spectra analysis confirmed that both of these fractions were mainly composed of galacturonic acid, arabinose, galactose and rhamnose, which were pectin-type polysaccharides with non-triple-helix structure. WSRP-2a and WSRP-2b, however, differed in molecular weight of 56.8 kD and 23.9 kD. The followed bioassay presented their impressive hypoglycemic and immunomodulatory activities. WSRP-2a promoted more proliferation, NO release, and the secretion of cytokines in RAW264.7 macrophages, while WSRP-2b presented higher α-amylase and α-glucosidase inhibitory activities. Collectively, these results suggested that the Rosa Setate x Rosa Rugosa waste biomass could be used as a promising source of bioactive pectic polysaccharides. |
| Databáze: | OpenAIRE |
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