Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study
| Autor: | Françoise Le Marrec-Croq, Timea Spakova, Isabelle Fournier, Julien Franck, Ján Rosocha, Stephanie Devaux, Juraj Blasko, Michel Salzet, Dasa Cizkova, Lucia Slovinska, Christophe Lefebvre |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Chemokine Stromal cell Bone Marrow Cells Article stomatognathic system Neurotrophic factors medicine Cytotoxic T cell Animals Immunologic Factors Rats Wistar Cells Cultured Multidisciplinary biology Microglia business.industry Chemotaxis Cell biology Rats medicine.anatomical_structure Culture Media Conditioned Immunology biology.protein Intercellular Signaling Peptides and Proteins Bone marrow Stromal Cells business Neurotrophin |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | In the present paper we develop a new non-cell based (cell-free) therapeutic approach applied to BV2 microglial cells and spinal cord derived primary microglia (PM) using conditioned media from rat bone marrow stromal cells (BMSCs-CM). First we collected conditioned media (CM) from either naive or injured rat spinal cord tissue (SCI-CM, inflammatory stimulation agent) and from rat bone marrow stromal cells (BMSCs-CM, therapeutic immunomodulation agent). They were both subsequently checked for the presence of chemokines and growth, neurotrophic and neural migration factors using proteomics analysis. The data clearly showed that rat BMSCs-CM contain in vitro growth factors, neural migration factors, osteogenic factors, differentiating factors and immunomodulators, whereas SCI-CM contain chemokines, chemoattractant factors and neurotrophic factors. Afterwards we determined whether the BMSCs-CM affect chemotactic activity, NO production, morphological and pro-apoptotic changes of either BV2 or PM cells once activated with SCI-CM. Our results confirm the anti-migratory and NO-inhibitory effects of BMSCs-CM on SCI-CM-activated microglia with higher impact on primary microglia. The cytotoxic effect of BMSCs-CM occurred only on SCI-CM-stimulated BV2 cells and PM, not on naive BV2 cells, nor on PM. Taken together, the molecular cocktail found in BMSCs-CM is favorable for immunomodulatory properties. |
| Databáze: | OpenAIRE |
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