A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life
| Autor: | Wu, Sheng-Yi, Wen, Yurong, Serre, Nelson B. C., Laursen, Cathrine Charlotte Heiede, Dietz, Andrea Grostøl, Taylor, Brian R., Drobizhev, Mikhail, Molina, Rosana S., Aggarwal, Abhi, Rancic, Vladimir, Becker, Michael, Ballanyi, Klaus, Podgorski, Kaspar, Hirase, Hajime, Nedergaard, Maiken, Fendrych, Matyáš, Lemieux, M. Joanne, Eberl, Daniel F., Kay, Alan R., Campbell, Robert E., Shen, Yi |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
Ions
Mice Fluorescence Resonance Energy Transfer/methods General Immunology and Microbiology Biosensing Techniques/methods General Neuroscience Fluorescence Resonance Energy Transfer Potassium Animals Biosensing Techniques General Agricultural and Biological Sciences General Biochemistry Genetics and Molecular Biology |
| Zdroj: | Wu, S-Y, Wen, Y, Serre, N B C, Laursen, C C H, Dietz, A G, Taylor, B R, Drobizhev, M, Molina, R S, Aggarwal, A, Rancic, V, Becker, M, Ballanyi, K, Podgorski, K, Hirase, H, Nedergaard, M, Fendrych, M, Lemieux, M J, Eberl, D F, Kay, A R, Campbell, R E & Shen, Y 2023, ' A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life ', PLOS Biology, vol. 20, no. 9, e3001772 . https://doi.org/10.1371/journal.pbio.3001772 |
| Popis: | Potassium ion (K+) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically-encoded fluorescent K+ biosensor, GINKO1, in the K+-bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K+ dynamics in multiple model organisms, including bacteria, plants, and mice. |
| Databáze: | OpenAIRE |
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