Feasibility of islet magnetic resonance imaging using ferumoxytol in intraportal islet transplantation
| Autor: | Jin Myung Choi, Wooyoung Shim, Seung-Hoon Oh, Dongkyeom Yoo, Jung Hee Lee, Sang-Man Jin, Jae Hyeon Kim, Dong Yun Lee, Yong Hwa Hwang, Bae Jun Oh |
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| Rok vydání: | 2014 |
| Předmět: |
Male
endocrine system Pathology medicine.medical_specialty Materials science endocrine system diseases Cell Survival Iron Biophysics Islets of Langerhans Transplantation Contrast Media Metal Nanoparticles Bioengineering Ferric Compounds Biomaterials Islets of Langerhans Magnetics Mice Microscopy Electron Transmission In vivo medicine Animals Protamines geography geography.geographical_feature_category Microscopy Confocal medicine.diagnostic_test business.industry Ultrasmall superparamagnetic iron oxide Heparin Magnetic resonance imaging Islet Magnetic Resonance Imaging Ferrosoferric Oxide Transplantation Ferumoxytol Mice Inbred C57BL Transplantation Isogeneic Mechanics of Materials Iron content Ceramics and Composites Nuclear medicine business Ex vivo Ferrocyanides |
| Zdroj: | Biomaterials. 52 |
| ISSN: | 1878-5905 |
| Popis: | There is a clinical need for an alternative labeling agent for magnetic resonance imaging (MRI) in islet transplantation. We aimed to evaluate the feasibility of islet MRI using ferumoxytol, which is the only clinically-available ultrasmall superparamagnetic iron oxide. We compared islet function and viability of control islets and islets labeled with ferumoxytol and/or a heparin-protamine complex (HPF). Efficacy of ferumoxytol labeling was assessed in both ex vivo and in vivo models. Labeling for 48 h with HPF, but not up to 800 μg/mL ferumoxytol, deranged ex vivo islet viability and function. The T2∗ relaxation time was optimal when islets were labeled with 800 μg/mL of ferumoxytol for 48 h. Prussian blue stain, iron content assay, transmission electron microscopy (TEM) supported internalization of ferumoxytol particles. However, the labeling intensity in the ex vivo MRI of islets labeled with ferumoxytol was much weaker than that of islets labeled with ferucarbotran. In syngeneic intraportal islet transplantation, there was a correlation between the total area of visualized islets and the transplanted islet mass. In conclusion, islet MRI using ferumoxytol was feasible in terms of in vitro and in vivo efficacy and safety. However, the weak labeling efficacy is still a hurdle for the clinical application. |
| Databáze: | OpenAIRE |
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