Determination of degree of RBC agglutination for blood typing using a small quantity of blood sample in a microfluidic system
| Autor: | Ching Yuan Ho, Yaw Jen Chang, Xin Miao Zhou, Hsiu Rong Yen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Agglutination
Erythrocytes Blood transfusion medicine.medical_treatment Microfluidics Biomedical Engineering Biophysics Analytical chemistry Biosensing Techniques 02 engineering and technology RBC Agglutination 01 natural sciences Blood typing Electrochemistry medicine Humans Chemistry 010401 analytical chemistry General Medicine 021001 nanoscience & nanotechnology Hematologic Diseases Power parameter 0104 chemical sciences Charge transfer resistance Red blood cell medicine.anatomical_structure Blood Grouping and Crossmatching Critical test 0210 nano-technology Biotechnology Biomedical engineering |
| Zdroj: | Biosensors and Bioelectronics. 102:234-241 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2017.11.008 |
| Popis: | Blood typing assay is a critical test to ensure the serological compatibility of a donor and an intended recipient prior to a blood transfusion. This paper presents a microfluidic blood typing system using a small quantity of blood sample to determine the degree of agglutination of red blood cell (RBC). Two measuring methods were proposed: impedimetric measurement and electroanalytical measurement. The charge transfer resistance in the impedimetric measurement and the power parameter in the electroanalytical measurement were used for the analysis of agglutination level. From the experimental results, both measuring methods provide quantitative results, and the parameters are linearly and monotonically related to the degree of RBC agglutination. However, the electroanalytical measurement is more reliable than the impedimetric technique because the impedimetric measurement may suffer from many influencing factors, such as chip conditions. Five levels from non-agglutination (level 0) to strong agglutination (level 4+) can be discriminated in this study, conforming to the clinical requirement to prevent any risks in transfusion. |
| Databáze: | OpenAIRE |
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