Toxicity of plant extracts containing pyrrolizidine alkaloids using alternative invertebrate models
| Autor: | Carmen Purdel, Denisa Margina, Michael D. Coleman, Cristina Elena Zbarcea, George Mihai Nitulescu, Octavian Tudorel Olaru, Demetrios A. Spandidos, Claudia Maria Gutu, Oana Cristina Seremet, Mihaela Ilie, Simona Negres, Aristides M. Tsatsakis |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Daphnia magna acute toxicity Biochemistry Median lethal dose Gas Chromatography-Mass Spectrometry Inhibitory Concentration 50 pyrrolizidine alkaloids 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Toxicity Tests Genetics Symphytum officinale Animals Senecio vernalis Molecular Biology GC-MS analysis Plants Medicinal biology Traditional medicine Plant Extracts Articles biology.organism_classification Invertebrates Artemia salina 030104 developmental biology Tussilago Oncology chemistry 030220 oncology & carcinogenesis Pyrrolizidine Toxicity Molecular Medicine medicinal plants |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | Pyrrolizidine alkaloids (PAs) are a widespread class of hepatotoxic heterocyclic organic compounds found in approximately 3% of world flora. Some PAs have been shown to have genotoxic and carcinogenic effects. The present study focuses on the toxicity effects of four dry extracts obtained from medicinal plants (Senecio vernalis, Symphytum officinale, Petasites hybridus and Tussilago farfara), on two aquatic organisms, Artemia salina and Daphnia magna, and the correlation with their PAs content. A new GC‑MS method, using a retention time (TR)‑5MS type capillary column was developed. PAs Kovats retention indices, for this type of column were computed for the first time. The lethal dose 50% (LC50) values for the two invertebrate models were correlated (Pearson 's coefficient, >0.9) and the toxicity was PA concentration-dependent, for three of the four extracts. All tested extracts were found to be toxic in both aquatic organism models. The results can be used to develop a GC‑MS validated method for the assay of PAs in medicinal plants with a further potential application in the risk assessment study of PAs toxicity in humans. |
| Databáze: | OpenAIRE |
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