Aging alters gene expression of growth and remodeling factors in human skeletal muscle both at rest and in response to acute resistance exercise
| Autor: | Charlotte A. Peterson, Patrick Kortebein, Pippa Simpson, Richard A. Dennis, Cathy M. Gurley, Beata Przybyla, Dennis H. Sullivan |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male medicine.medical_specialty Aging Weight Lifting Physiology Rest Muscle Proteins Inflammation Myostatin Article Transforming Growth Factor beta Internal medicine Gene expression Genetics medicine Humans Ciliary Neurotrophic Factor RNA Messenger Insulin-Like Growth Factor I Muscle Skeletal Actin Aged Regulation of gene expression Tissue Inhibitor of Metalloproteinase-1 biology Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Skeletal muscle Transforming growth factor beta Actins Gene expression profiling medicine.anatomical_structure Endocrinology Gene Expression Regulation biology.protein Intercellular Signaling Peptides and Proteins Matrix Metalloproteinase 2 medicine.symptom Insulin-Like Growth Factor Binding Protein 5 |
| Zdroj: | Physiological genomics. 32(3) |
| ISSN: | 1531-2267 |
| Popis: | Aging alters gene expression of growth and remodeling factors in human skeletal muscle both at rest and in response to acute resistance exercise. Physiol Genomics 32: 393–400, 2008. First published December 11, 2007; doi:10.1152/physiolgenomics.00191.2007.—The purpose of this investigation was to compare expression of genes that function in inflammation and stress, cell structure and signaling, or remodeling and growth in skeletal muscle of young (32 ± 7 yr, n =15) and elderly (72 ±5 yr, n = 16) healthy subjects before and after a bout of resistance leg exercises. A real-time RT-PCR method was used to screen 100 transcripts in v. lateralis biopsies obtained before and 72 h postexercise. The screen identified 15 candidates for differential expression due to aging and/or exercise that were measured quantitatively. The median levels of four mRNAs (insulin-like growth factor-1 and its binding protein IGFBP5, ciliary neurotrophic factor, and the metallopeptidase MMP2) were significantly affected by aging and were greater (1.6- to 2.3-fold, P ≤0.05) in the young than elderly muscle at both time points. The median levels of three mRNAs were significantly (P ≤0.05) affected by exercise in the young. The metallopeptidase inhibitor TIMP1 and [H9251]-cardiac actin mRNAs increased 2-fold and 6.5-fold, respectively, and GDF8 (myostatin) mRNA decreased by 50%. However, elderly muscle did not display any significant changes in gene expression postexercise. Thus, aging muscle shows decreased levels at rest and an impaired response to exercise for a number of mRNAs for factors potentially involved in muscle growth and remodeling. Future studies must determine the functional importance of these gene expression changes to protein synthesis, satellite cell activity, and other processes that are directly involved in the mechanisms of muscle hypertrophy. |
| Databáze: | OpenAIRE |
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