Simplified Method to Measure Glomerular Filtration Rate by Iohexol Plasma Clearance in Conscious Rats
| Autor: | Matteo Fois, Nadia Stucchi, Beatriz Abrante, Daniela Corna, Sergio Luis-Lima, Domenico Cerullo, Esteban Porrini, Sebastian Villa, Silvia Ferrari, Flavio Gaspari, Giovanni Nattino, Carlamaria Zoja, Fabiola Carrara, Antonio Cannata, Nadia Azzollini |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty Iohexol 030232 urology & nephrology Measure (physics) Urology Renal function 030204 cardiovascular system & hematology urologic and male genital diseases Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Animals Medicine Plasma clearance urogenital system business.industry female genital diseases and pregnancy complications Rats Filtration fraction Rats Inbred Lew Female business Glomerular Filtration Rate Clearance medicine.drug |
| Zdroj: | Nephron. 133:62-70 |
| ISSN: | 2235-3186 1660-8151 |
| DOI: | 10.1159/000445843 |
| Popis: | Background/Aims: Glomerular filtration rate (GFR) is the best index for evaluating renal function. We aimed to develop a simplified iohexol plasma clearance procedure for GFR measurement in rats without urine collection, animal catheterization or anesthesia, with limited sampling and requiring blood instead of plasma, to further reduce the sample volume and improve animal welfare. Methods: After iohexol injection (129.4 mg), samples were drawn according to 2-compartment kinetics and analyzed by high performance liquid chromatography. Healthy male Lewis rats were used to find a correction factor (CF) to obtain the ‘reference clearance' from the simplified 1-comparment model. This approach was validated using male or female (Lewis, Sprague-Dawley) rats and animals with renal mass reduction (RMR). In additional rats, different simplified approaches were evaluated. Results: Iohexol concentrations in blood and plasma strongly correlated (r = 0.9784, p < 0.0001). A CF of 0.90 enabled the calculation of the reference GFR. Validation results in male Lewis rats were 0.99 ± 0.27 for the reference GFR and 1.03 ± 0.29 ml/min/100 g for the simplified approach. Results in female Sprague-Dawley rats confirmed the suitability of the proposed method. In RMR rats, GFR was 0.14 ± 0.05 and 0.14 ± 0.04 ml/min/100 g for the reference and simplified model, respectively. Conclusion: The procedure we set up to measure GFR in conscious rats was proven to be reliable, required a small volume of blood at only 4 selected time points, without the need to collect urine or catheterize the animals, was applicable to rats from different strains and sexes, both healthy and with renal function impairment. Moreover, the procedure enables the monitoring of GFR changes over time in the same animal, thereby reducing the number of animals to be used. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|