Chemiluminescence and reactive oxygen scavenging activities of the hydrogen peroxide/gallic acid/lactoperoxidase system
| Autor: | Masami Kawanari, Yoshinobu Akiyama, Yumiko Yoshiki, Kaoru Sato, Kazuyoshi Okubo |
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| Rok vydání: | 2000 |
| Předmět: |
Inorganic chemistry
Biophysics chemistry.chemical_element In Vitro Techniques Oxygen law.invention Linoleic Acid chemistry.chemical_compound Superoxides law Gallic Acid Animals Lactoperoxidase Gallic acid Hydrogen peroxide Scavenging Chemiluminescence chemistry.chemical_classification Reactive oxygen species Superoxide Free Radical Scavengers Hydrogen Peroxide Hydrogen-Ion Concentration chemistry Chemistry (miscellaneous) Luminescent Measurements Thermodynamics Cattle Oxidation-Reduction |
| Zdroj: | Luminescence. 15:183-187 |
| ISSN: | 1522-7243 1522-7235 |
| DOI: | 10.1002/1522-7243(200005/06)15:3<183::aid-bio584>3.0.co;2-v |
| Popis: | Photon emission in the hydrogen peroxide/gallic acid/lactoperoxidase system was studied using visible region chemiluminescence. The photon emission intensity showed a pH-dependence curve with a maximum at pH 4.5–5.0. The spectral analysis showed to be at 510 nm and its energy was 61.0 kcal/mol at either pH 4.5 or pH 7.0. In addition, near-infrared spectral analysis at 1270 nm suggested that this photon emission from the hydrogen peroxide/gallic acid/lactoperoxidase system was produced without generation of 1O2. The gallic acid/lactoperoxidase system, based on the chemiluminescence system, has superoxide-, hydroxyl radical-scavenging activities and antioxidative activity. These results are strong evidence that the gallic acid/lactoperoxidase system is one of the reactive oxygen scavenging systems. © 2000 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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