Antimicrobial photodynamic efficiency of novel cationic porphyrins towards periodontal Gram-positive and Gram-negative pathogenic bacteria
| Autor: | Vanya Mantareva, Latchezar Avramov, Leslie Selvaraj, Danaboyina Ramaiah, Ivan Angelov, Suneesh C. Karunakaran, Albish K. Paul, Krishnankutty Nandakumar, Chandra Sekhar Prasanth, Narayanan Subhash, Vesselin Kussovski |
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| Rok vydání: | 2013 |
| Předmět: |
Periodontium
Magnetic Resonance Spectroscopy Porphyrins medicine.medical_treatment Photodynamic therapy medicine.disease_cause Gram-Positive Bacteria Biochemistry Microbiology chemistry.chemical_compound Cations Gram-Negative Bacteria polycyclic compounds medicine Physical and Theoretical Chemistry Photosensitizing Agents biology Singlet oxygen Aggregatibacter actinomycetemcomitans Pathogenic bacteria General Medicine biology.organism_classification Antimicrobial Porphyrin chemistry Photochemotherapy Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Fusobacterium nucleatum Bacteria |
| Zdroj: | Photochemistry and photobiology. 90(3) |
| ISSN: | 1751-1097 |
| Popis: | The Gram-negative Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum are major causative agents of aggressive periodontal disease. Due to increase in the number of antibiotic-resistant bacteria, antimicrobial Photodynamic therapy (aPDT) seems to be a plausible alternative. In this work, photosensitization was performed on Gram-positive and Gram-negative bacteria in pure culture using new-age cationic porphyrins, namely mesoimidazolium-substituted porphyrin derivative (ImP) and pyridinium-substituted porphyrin derivative (PyP). The photophysical properties of both the sensitizers including absorption, fluorescence emission, quantum yields of the triplet excited states and singlet oxygen generation efficiencies were evaluated in the context of aPDT application. The studied porphyrins exhibited high ability to accumulate into bacterial cells with complete penetration into early stage biofilms. As compared with ImP, PyP was found to be more effective for photoinactivation of bacterial strains associated with periodontitis, without any signs of dark toxicity, owing to its high photocytotoxicity. |
| Databáze: | OpenAIRE |
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