Neural circuit analysis using a novel intersectional split intein-mediated split-Cre recombinase system
| Autor: | Ho Min Kim, Audrey Tze Ting Khoo, Hyunsoo Shawn Je, Paul Jong Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Retrograde virus Transgene Green Fluorescent Proteins Cre recombinase Mice Transgenic Computational biology Biology lcsh:RC346-429 Inteins 03 medical and health sciences Cellular and Molecular Neuroscience GABA 0302 clinical medicine Transgenic mouse Gene expression Recombinase medicine Biological neural network Circuit mapping Animals Humans GABAergic Neurons Luciferases Molecular Biology DLX6 lcsh:Neurology. Diseases of the nervous system Projection neurons Integrases Methodology Reproducibility of Results Split intein 030104 developmental biology medicine.anatomical_structure HEK293 Cells Neuron Nerve Net Intein 030217 neurology & neurosurgery Split-cre recombinase Network analysis |
| Zdroj: | Molecular Brain Molecular Brain, Vol 13, Iss 1, Pp 1-10 (2020) |
| ISSN: | 1756-6606 |
| Popis: | The defining features of a neuron are its functional and anatomical connections with thousands of other neurons in the brain. Together, these neurons form functional networks that direct animal behaviour. Current approaches that allow the interrogation of specific populations of neurons and neural circuits rely heavily on targeting their gene expression profiles or connectivity. However, these approaches are often unable to delineate specific neuronal populations. Here, we developed a novel intersectional split intein-mediated split-Cre recombinase system that can selectively label specific types of neurons based on their gene expression profiles and structural connectivity. We developed this system by splitting Cre recombinase into two fragments with evolved split inteins and subsequently expressed one fragment under the influence of a cell type-specific promoter in a transgenic animal, and delivered the other fragment via retrograde viral gene transfer. This approach results in the reconstitution of Cre recombinase in only specific population of neurons projecting from a specific brain region or in those of a specific neuronal type. Taken together, our split intein-based split-Cre system will be useful for sophisticated characterization of mammalian brain circuits. |
| Databáze: | OpenAIRE |
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