Electrochemical Determination of Glutathione in Plasma at Carbon Nanotubes Based Screen Printed Electrodes
| Autor: | Arzum Erdem, Guliz Armagan, Hakan Karadeniz, Ayfer Yalcin, Ezgi Turunc |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Antioxidant
medicine.medical_treatment Endogeny Electrochemistry medicine.disease_cause chemistry.chemical_compound Limit of Detection Drug Discovery medicine Animals Trichloroacetic acid Electrodes Detection limit Chromatography Nanotubes Carbon Organic Chemistry Electrochemical Techniques Equipment Design General Medicine Glutathione Rats Computer Science Applications chemistry Biochemistry Differential pulse voltammetry Oxidative stress |
| Zdroj: | Combinatorial Chemistry & High Throughput Screening. 16:695-701 |
| ISSN: | 1386-2073 |
| DOI: | 10.2174/13862073113169990040 |
| Popis: | Glutathione (GSH) is a major endogenous antioxidant highly active in human tissues and plays a key role in controlling cellular thiol redox system, maintaining the immune and detoxification system. The determination of GSH levels in tissue is important to estimate endogenous defenses against oxidative stress. In our study, the multi-walled carbon nanotube modified screen-printed electrodes (MWCNT-SPEs) were used to determine the levels of GSH in trichloroacetic acid (TCA)-treated or untreated samples of rat plasma. It was found that the deproteinization of samples with TCA improved the electrochemical detection of GSH particularly in plasma. The oxidation of GSH was measured by using differential pulse voltammetry (DPV) method in combination with MWCNT-SPE (n=3), and the detection limit of GSH was found to be 0.47 mu M (S/N=3). The GSH levels in plasma samples were also measured spectrophotometrically in order to compare the effectiveness of electrochemical method and we obtained a high correlation between the two methods (R-2=0.976). |
| Databáze: | OpenAIRE |
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