Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst
| Autor: | Akihiko Kondo, Kentaro Kiriyama, Hideki Fukuda, Fumio Matsuda, Chiaki Ogino, Hideyo Yoshida, Kiyotaka Y. Hara, Hideki Nakayama, Fumiyoshi Okazaki |
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| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
biology Saccharomyces cerevisiae General Medicine Glutathione biology.organism_classification Applied Microbiology and Biotechnology Bioproduction Yeast Enzymes Metabolic engineering chemistry.chemical_compound Enzyme Adenosine Triphosphate chemistry Biochemistry Biocatalysis Whole cell Genetic Engineering Metabolic Networks and Pathways Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 91(4) |
| ISSN: | 1432-0614 |
| Popis: | We developed a novel enzymatic glutathione (GSH) production system using Saccharomyces cerevisiae as a whole-cell biocatalyst, and improved its GSH productivity by metabolic engineering. We demonstrated that the metabolic engineering of GSH pathway and ATP regeneration can significantly improve GSH productivity by up to 1.7-fold higher compared with the parental strain, respectively. Furthermore, the combination of both improvements in GSH pathway and ATP regeneration is more effective (2.6-fold) than either improvement individually for GSH enzymatic production using yeast. The improved whole-cell biocatalyst indicates its great potential for applications to other kinds of ATP-dependent bioproduction. |
| Databáze: | OpenAIRE |
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