Immune Response in Patients Receiving a Bioprosthetic Heart Valve: Lack of Response with Decellularized Valves
| Autor: | Wilhelm Erdbrügger, Pia Golde, Pascal M. Dohmen, Oliver Bloch, Wolfgang Konertz, Steffen Posner |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Sus scrofa Biomedical Engineering Bioengineering Biochemistry Collagen Type I Immunoglobulin G Biomaterials Immune system medicine Animals Humans In patient Heart valve Enzyme Assays Bioprosthesis Decellularization biology business.industry Immunity Antibody titer Middle Aged medicine.disease medicine.anatomical_structure Glutaral Heart Valve Prosthesis alpha-Galactosidase Immunology biology.protein Cattle Female Antibody business Calcification |
| Zdroj: | Tissue Engineering Part A. 17:2399-2405 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2011.0046 |
| Popis: | Conventional biological heart valves treated with glutaraldehyde (GA) reveal a limited lifespan due to calcification. This is assumed to be an immune response initiated process, which is not seen with decellularized valves. However, their immunological potential is still a matter of debate. Therefore, serum samples from patients undergoing heart valve surgery were obtained before (Pre), after (Post), and 9-12 months after operation (Follow Up). Immunoglobulin G (IgG) and M (IgM) antibodies against porcine collagen I and α-Gal (Gal-alpha1,3-Gal-beta1,4-GlcNac-R) were determined for decellularized and GA treated valves. Antibody titers for collagen type I revealed no significant alteration for both types of valves. However, a considerable anti-α-Gal antibody response was observed in patients with GA-treated porcine valves. In detail, IgM antibodies were increased during follow up (p0.05), whereas decellularized valves revealed a minor decrease in the IgM response (p0.001). IgG antibodies were considerably increased with GA-treated porcine (p0.05) and bovine (p0.01) xenografts, whereas there was lack of response with decellularized valves. This indicates that GA treatment is not sufficient to eliminate immune response to the α-Gal epitope completely. Future investigations will have to verify whether immune response to α-Gal can be linked to the limited durability of conventional valves. |
| Databáze: | OpenAIRE |
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