In vitro evaluation of co-exposure of arsenium and an organic nanomaterial (fullerene, C60) in zebrafish hepatocytes

Autor: Leandro Machado de Carvalho, Carmen L. Azevedo Costa, Josencler L. Ribas Ferreira, José Maria Monserrat, Juliane Ventura-Lima, Luis Ferraz, Isabel Soares Chaves
Rok vydání: 2012
Předmět:
Zdroj: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 155(2):206-212
ISSN: 1532-0456
DOI: 10.1016/j.cbpc.2011.08.005
Popis: Taking into account the concept of the “Trojan Horse”, where contaminants may have its entry into specific organs potentiated by its association with nanomaterials, the aim of this study was to analyze the joint toxic effects induced by an organic nanomaterial, fullerene (C 60 ) with the metalloid arsenic (As III ). Hepatocytes of zebrafish Danio rerio were exposed to As III (2.5 or 100 μM), C 60 or As + C 60 for 4 h, not altering cells viability. Intracellular reactive oxygen species concentration was reduced in cells exposed only to the C 60 (1 mg/L) and in the treatment of 100 μM As III + C 60 . Co-exposure with C 60 abolished the peak of the antioxidant glutathione (GSH) registered in cells exposed to the lowest As III concentration (2.5 μM). A similar result was observed in terms of lipid damage (TBARS). Total antioxidant capacity was significantly higher at both As III concentrations co-exposed to C 60 when compared with the control group. Activity of glutathione-S-transferase omega, a limiting enzyme in the methylation pathway of As III , was reduced in the 100 μM As III + C 60 treatment. Cells co-exposed to C 60 had a significantly higher accumulation of As III , showing a “Trojan Horse” effect which did not result in higher cell toxicity. Instead, co-exposure of As III with C 60 showed to reduce cellular injury.
Databáze: OpenAIRE
Externí odkaz: