Pins is not required for spindle orientation in the Drosophila wing disc
| Autor: | Nicole S Dawney, Holly E. Lovegrove, Daniel St Johnston, Izabela Kujawiak, Jinwei Zhu, Daniel T. Bergstralh, Rongguang Zhang, Samantha Cooper |
|---|---|
| Přispěvatelé: | Bergstralh, Dan T [0000-0003-1689-3715], St Johnston, Daniel [0000-0001-5582-3301], Apollo - University of Cambridge Repository |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
animal structures Dynein Cell Cycle Proteins Spindle Apparatus Biology 03 medical and health sciences Mud/NuMA Pins/LGN Animals Drosophila Proteins Wings Animal Prometaphase Molecular Biology Mitosis Metaphase Guanine Nucleotide Dissociation Inhibitors fungi Anatomy Spindle orientation Spindle apparatus Cell biology Cytoskeletal Proteins Imaginal disc Drosophila melanogaster 030104 developmental biology Imaginal Discs Mutation Dynactin Drosophila Epithelia Astral microtubules Cell Division Research Article Signal Transduction Developmental Biology |
| Zdroj: | Bergstralh, D T, Lovegrove, H, Kujawiak, I, Dawney, N S, Zhu, J, Cooper, S, Zhang, R & Johnston, D S 2016, ' Pins is not required for spindle orientation in the drosophila wing disc ', Development (Cambridge), vol. 143, no. 14, pp. 2573-2581 . https://doi.org/10.1242/dev.135475 Development (Cambridge, England) |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.135475 |
| Popis: | In animal cells, mitotic spindles are oriented by the dynein/dynactin motor complex, which exerts a pulling force on astral microtubules. Dynein/dynactin localization depends on Mud/NUMA, which is typically recruited to the cortex by Pins/LGN. In Drosophila neuroblasts, the Inscuteable/Baz/Par-6/aPKC complex recruits Pins apically to induce vertical spindle orientation, whereas in epithelial cells Dlg recruits Pins laterally to orient the spindle horizontally. Here we investigate division orientation in the Drosophila imaginal wing disc epithelium. Live imaging reveals that spindle angles vary widely during prometaphase and metaphase, and therefore do not reliably predict division orientation. This finding prompted us to re-examine mutants that have been reported to disrupt division orientation in this tissue. Loss of Mud misorients divisions, but Inscuteable expression and aPKC, dlg and pins mutants have no effect. Furthermore, Mud localizes to the apical-lateral cortex of the wing epithelium independently of both Pins and cell cycle stage. Thus, Pins is not required in the wing disc because there are parallel mechanisms for Mud localization and hence spindle orientation, making it a more robust system than in other epithelia. This work was supported by a Wellcome Trust Principal Fellowship to DStJ [080007] and by core support from the Wellcome Trust [092096] and Cancer Research UK [A14492]. DTB was supported by a Marie Curie Fellowship and the Wellcome Trust. HEL was supported by a Herchel Smith Studentship. This is the author accepted manuscript. The final version is available from The Company of Biologists via http://dx.doi.org/10.1242/dev.135475 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|