Ageing effects on the expression of cell defence genes after UVA irradiation in human male cutaneous fibroblasts using cDNA arrays

Autor: Jean-Claude Beani, Catherine Mouret, Kita Valenti, Florence Hazane, Sylvie Sauvaigo, André Peinnequin, Alain Favier
Přispěvatelé: Laboratoire Oligoéléments et Résistance au Stress Oxydant induit par les Xénobiotiques (Laboratoire ORSOX), Université Joseph Fourier - Grenoble 1 (UJF)-UMR-E3 UJF/CEA-LRC-CEA(LAN) 8M, Département de biologie intégrée, CHU Grenoble-Hôpital Michallon, Laboratoire Lésions des Acides Nucléiques (LAN), Service de Chimie Inorganique et Biologique (SCIB - UMR E3), Institut Nanosciences et Cryogénie (INAC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut Nanosciences et Cryogénie (INAC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS), Département de Radiobiologie et Radiopathologie, Service de Santé des Armées-Ministère de la Défense, Service de dermatologie, Sinniger, Valérie
Rok vydání: 2005
Předmět:
Male
Transcription
Genetic
MMP1
Photoaging
Cell
medicine.disease_cause
MESH: Aged
80 and over
0302 clinical medicine
Gene expression
Cells
Cultured
Oligonucleotide Array Sequence Analysis
Aged
80 and over
MESH: Aged
0303 health sciences
MESH: Oxidative Stress
Radiation
[SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology
Radiological and Ultrasound Technology
[SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology
MESH: Gene Expression Regulation
MESH: Internet
medicine.anatomical_structure
Child
Preschool
030220 oncology & carcinogenesis
MESH: Cells
Cultured
Adult
Ultraviolet Rays
DNA repair
Biophysics
Biology
03 medical and health sciences
medicine
Humans
Radiology
Nuclear Medicine and imaging
Gene
Aged
030304 developmental biology
Internet
MESH: Humans
MESH: Transcription
Genetic
MESH: Child
Preschool
MESH: Adult
Fibroblasts
medicine.disease
Molecular biology
MESH: Male
Skin Aging
Oxidative Stress
MESH: Cytoprotection
Gene Expression Regulation
Cytoprotection
MESH: Fibroblasts
Ageing
MESH: Oligonucleotide Array Sequence Analysis
MESH: Skin Aging
MESH: Ultraviolet Rays
Oxidative stress
Zdroj: Journal of Photochemistry and Photobiology B: Biology
Journal of Photochemistry and Photobiology B: Biology, Elsevier, 2005, 79 (3), pp.171-90. ⟨10.1016/j.jphotobiol.2005.02.001⟩
Journal of Photochemistry and Photobiology B: Biology, 2005, 79 (3), pp.171-90. ⟨10.1016/j.jphotobiol.2005.02.001⟩
ISSN: 1011-1344
Popis: International audience; Ageing is a multifactorial process in which reactive oxygen species (ROS) are thought to be implicated. ROS cause oxidative alterations on cell constituents, and damage accumulation can lead to mutations in DNA. Modulation of gene expression during ageing is now quite documented but results are often controversial and/or incomplete. As ultraviolet A is one of the exogenous factors involved in skin ageing, by the production of ROS, we further document the modifications in gene expression during ageing process and response to an oxidative stress. For this purpose, we used a cDNA macroarray containing 82 genes related to cell defence, essentially represented by antioxidant and DNA repair proteins. Ageing-associated gene expression was assessed in normal skin human fibroblasts from three age groups: children (n=4), adults (n=4) and olders (n=3), at the basal state and after a 5J/cm2 UVA irradiation. Analysis revealed that 22 genes were never detected, whereas certain were always expressed such as those related to antioxidant defence, extracellular matrix (ECM) regulator and XPC. Transcripts related to ECM, MMP1 and MMP3 were increased with age and after UVA irradiation, independently of age. It appeared that transcripts involved in the redox status control (TXN and APEX) decreased as a function of age, at the basal state and after irradiation, respectively. Most of transcripts involved in DNA repair were not detected but repression of POLD1 in the adult group and induction of XRCC5 and LIG4 were observed after UVA irradiation, as a function of age. In the basal state, the transcript of GAS1, regulator of cell cycle arrest in G1 phase was found to be decreased with age. HMOX1 increased after UVA irradiation. In conclusion, the decrease in expression of some antioxidant system, cell cycle control gene and extracellular matrix enzymes, particularly after UV exposure can explain the occurrence of photoaging.
Databáze: OpenAIRE
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