Analysis of TATA-binding protein 2 (TBP2) and TBP expression suggests different roles for the two proteins in regulation of gene expression during oogenesis and early mouse development
| Autor: | Emese Gazdag, Aleksandar Rajkovic, Laszlo Tora, Maria-Elena Torres-Padilla |
|---|---|
| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Embryology
Transcription Genetic MESH: 3T3 Cells Fluorescent Antibody Technique Gene Expression MESH: TATA-Box Binding Protein Oogenesis MESH: Mice Knockout MESH: Antibodies Monoclonal Mice 0302 clinical medicine Endocrinology Ovarian Follicle MESH: Gene Expression Regulation Developmental Transcriptional regulation MESH: Embryonic Development MESH: Animals MESH: Fluorescent Antibody Technique Fertilisation Mice Knockout Regulation of gene expression 0303 health sciences education.field_of_study Antibodies Monoclonal Gene Expression Regulation Developmental Obstetrics and Gynecology 3T3 Cells MESH: Oogenesis Cell biology medicine.anatomical_structure Female Folliculogenesis Nucleoplasmin MESH: Gene Expression Blotting Western Embryonic Development Biology Transfection MESH: Oocytes 03 medical and health sciences MESH: Mice Inbred C57BL medicine Animals MESH: Blotting Western education MESH: Mice 030304 developmental biology MESH: Transcription Genetic MESH: Transfection MESH: TATA Box Binding Protein-Like Proteins [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology TATA-Box Binding Protein Oocyte Molecular biology MESH: Ovarian Follicle Mice Inbred C57BL Reproductive Medicine Oocytes biology.protein TATA Box Binding Protein-Like Proteins TATA-binding protein MESH: Female 030217 neurology & neurosurgery |
| Zdroj: | Reproduction Reproduction, BioScientifica, 2007, 134 (1), pp.51-62. ⟨10.1530/REP-06-0337⟩ |
| ISSN: | 1470-1626 1741-7899 |
| DOI: | 10.1530/REP-06-0337⟩ |
| Popis: | Gametogenesis, the process during which germ cells are generated is essential for reproduction. In mammals, maternal mRNA and proteins present in the oocyte are required to ensure the progression of development until the embryo activates its genome after fertilisation. It is well established that the oocyte synthesises these maternal factors during oocyte growth and then undergoes a quiescent transcriptional period that will be resumed only after fertilisation. However, the mechanisms that govern transcriptional regulation and subsequent silencing during oogenesis are not well understood. Here, we have examined the expression and localisation of the TATA-binding protein (TBP) and the related protein TBP2 (also called TRF3, TBP-related factor 3) during oogenesis and in early mouse embryos. We show that TBP is expressed in the oocytes at the beginning of folliculogenesis, but it is undetectable during further stages of oocyte development, and becomes abundant again only after fertilisation. In contrast to TBP, we found that TBP2 is highly expressed in growing oocytes during folliculogenesis, declines upon ovulation, and is almost undetectable after fertilisation by the two-cell stage. The mirroring localisation profile of TBP and TBP2 suggests different roles for the two proteins in establishing specialised programs of gene expression during oocyte development and in early mouse embryos. Analysis of mutant mouse ovaries in which oocyte-specific factors have been knocked-out suggests that TBP2 is a potential candidate for regulating transcriptional control of oogenesis. Moreover, our results obtained with oocytes lacking the oocyte-specific nuclear chaperone nucleoplasmin 2 suggest that TBP2 function may be related to non-condensed chromatin conformation. |
| Databáze: | OpenAIRE |
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