The platelet‐derived growth factor receptor alpha promoter‐directed expression of cre recombinase in mouse placenta

Autor: David R. C. Natale, Jianhua Shao, Liping Qiao, Jean-Sebastien Wattez, Samuel Lee
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Developmental Dynamics
ISSN: 1097-0177
1058-8388
Popis: Background Numerous pathologies of pregnancy originate from placental dysfunction. It is essential to understand the functions of key genes in the placenta in order to discern the etiology of placental pathologies. A paucity of animal models that allow conditional and inducible expression of a target gene in the placenta is a major limitation for studying placental development and function. Results To study the platelet‐derived growth factor receptor alpha (PDGFRα)‐directed and tamoxifen‐induced Cre recombinase expression in the placenta, PDGFRα‐CreER mice were crossed with mT/mG dual‐fluorescent reporter mice. The expression of endogenous membrane‐localized enhanced green fluorescent protein (mEGFP) and/or dTomato in the placenta was examined to identify PDGFRα promoter‐directed Cre expression. Pregnant PDGFRα‐CreER;mT/mG mice were treated with tamoxifen at various gestational ages. Upon tamoxifen treatment, reporter protein mEGFP was observed in the junctional zone (JZ) and chorionic plate (CP). Furthermore, a single dose of tamoxifen was sufficient to induce the recombination. Conclusions PDGFRα‐CreER expression is restricted to the JZ and CP of mouse placentas. PDGFRα‐CreER mice provide a useful tool to conditionally knock out or overexpress a target gene in these regions of the mouse placenta.
Key Findings Inducible PDGFRα‐directed Cre expression trophoblasts cells.A single tamoxifen treatment is sufficient to induce the recombination.Valuable tool to temporary knockout or over‐express a target gene in the placenta.Do not require sophisticated system and suitable for ordinary laboratory setting.
Databáze: OpenAIRE
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