Effective prophylaxis of influenza A virus pneumonia in mice by topical passive immunotherapy with polyvalent human immunoglobulins or F(ab′)2 fragments
| Autor: | J M Alonso, F X Deramoudt, F Ramisse, Marek Szatanik, P Binder, C Hannoun, A Bianchi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Immunology
Orthomyxoviridae Pneumonia Viral Chick Embryo medicine.disease_cause Virus Immunoglobulin G Mice Dogs Orthomyxoviridae Infections hemic and lymphatic diseases Influenza Human Influenza A virus medicine Immunology and Allergy Animals Humans Immunoglobulin Fragments Mice Inbred BALB C biology business.industry Lethal dose Immunization Passive Immunoglobulins Intravenous Hemagglutination Inhibition Tests biology.organism_classification medicine.disease Virology Pneumonia biology.protein Nasal administration Original Article Female Antibody business |
| Popis: | SUMMARYThe effectiveness of polyvalent plasma-derived human immunoglobulins (IVIG) in passive immunotherapy of influenza virus pneumonia was assessed, using the Strain Scotland (A/Scotland/74 (H3N2)) adapted to BALB/c mice by repeated lung passages. Haemagglutinin antibodies in two batches of IVIG at 10 mg/ml had a titre of 1/16. Intravenous injection of 1000–5000 μg of IVIG, 3 h after infection, gave 60–70% protection, whereas intranasal injection of 25–50 μg protected 90% of mice infected with a lethal dose of influenza virus. F (ab′)2 fragments were at least as protective as intact IVIG, suggesting that complement or Fcγ receptor-bearing cells were not required. Topical passive immunotherapy with IVIG or F(ab′)2 gave protection up to 8 h after infection, but not at 24 h, suggesting that anti-influenza A antibodies in IVIG, delivered locally, are only effective at early stages of the infectious process. The potential value of topical administration of IVIG or F(ab′)2 fragments for influenza A pneumonia prophylaxis was further demonstrated by the protective effects of their intranasal administration 24 h before challenge. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|