Unstable visible mutations induced in Drosophila melanogaster by injections of oncogenic virus DNA into the polar plasm of early embryos
| Autor: | Goltzov Va, E. N. Shibanova, S. D. Nabirochkin, K G Gazaryan, A. G. Tatosyan, Ambartsumian Ns, Tikhonenko Ti, Kisselev Fl |
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| Rok vydání: | 1987 |
| Předmět: |
Cytoplasm
Embryo Nonmammalian Microinjections DNA Recombinant medicine.disease_cause Adenoviridae chemistry.chemical_compound Retrovirus Genetics medicine Animals Molecular Biology Southern blot Rous sarcoma virus Mutation biology Mutagenesis biology.organism_classification Molecular biology Drosophila melanogaster Phenotype chemistry Avian Sarcoma Viruses DNA Viral Pole plasm DNA Oncovirus |
| Zdroj: | Moleculargeneral genetics : MGG. 207(1) |
| ISSN: | 0026-8925 |
| Popis: | When RSV DNA cloned in pBR 322 or DNA of simian adenovirus Sa7 (C8) is injected into the pole plasm of embryos of various Drosophila stocks, the progeny of 1-70% of the surviving flies display visible mutations. The mutagenesis is partially directed: the loci mutating due to retrovirus and adenovirus DNA do not overlap. The majority of resulting mutants are characterised by high instability: reversions and new mutations occur in them, which sometimes spread over the whole population ("explosive" instability). The injected sequences are revealed by dot-hybridization in the DNA of many mutant strains, but only rarely by Southern blotting procedures. The results show that the microinjection of oncovirus DNA into embryos is an approach for obtaining highly unstable strains even from wild-type stable Drosophila stocks without crosses with MR lines or the introduction of P elements. The sets of unstable mutations induced by oncovirus DNA is different from those in hybrid dysgenesis. |
| Databáze: | OpenAIRE |
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