The Angiogenic Factor Angiopoietin-1 Is a Proneurogenic Peptide on Subventricular Zone Stem/Progenitor Cells
| Autor: | Liliana Bernardino, João O. Malva, Fabienne Agasse, Luísa Cortes, Joana Gonçalves, Alexandra Isabel Rosa |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
MAP Kinase Signaling System
Neurogenesis Cellular differentiation Subventricular zone Protein Serine-Threonine Kinases Cerebral Ventricles Mice Neurosphere Angiopoietin-1 medicine Animals RNA Messenger Progenitor cell Protein kinase A Cell Proliferation Neurons Cell Death biology Stem Cells TOR Serine-Threonine Kinases General Neuroscience Intracellular Signaling Peptides and Proteins Brain Cell Differentiation Articles Olfactory Bulb Receptor TIE-2 Axons Cell biology Mice Inbred C57BL medicine.anatomical_structure nervous system biology.protein NeuN Stem cell Neuroglia |
| Zdroj: | The Journal of Neuroscience. 30:4573-4584 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | In the adult mammalian brain, the subventricular zone (SVZ) hosts stem cells constantly generating new neurons. Angiopoietin-1 (Ang-1) is an endothelial growth factor with a critical role in division, survival, and adhesion of endothelial cells via Tie-2 receptor activity. Expression of Tie-2 in nonendothelial cells, especially neurons and stem cells, suggests that Ang-1 may be involved in neurogenesis. In the present work, we investigated the putative role of Ang-1 on SVZ neurogenesis. Immature cells from SVZ-derived neurospheres express Ang-1 and Tie-2 mRNA, suggesting a role for the Ang-1/Tie-2 system in the neurogenic niche. Moreover, we also found that Tie-2 protein expression is retained on differentiation in neurons and glial cells. Ang-1 triggered proliferation via activation of the ERK1/2 (extracellular signal-regulated kinase 1/2) mitogen-activated protein kinase (MAPK) kinase pathway but did not induce cell death. Accordingly, coincubation with an anti-Tie-2 neutralizing antibody prevented the pro-proliferative effect of Ang-1. Furthermore, Ang-1 increased the number of NeuN (neuronal nuclear protein)-positive neurons in cultures treated for 7 d, as well as the number of functional neurons, as assessed by monitoring [Ca2+]irises after application of specific stimuli for neurons and immature cells. The proneurogenic effect of Ang-1 is mediated by Tie-2 activation and subsequent mTOR (mammalian target of rapamycin kinase) mobilization. In agreement, neuronal differentiation significantly decreased after exposure to an anti-Tie-2 neutralizing antibody and to rapamycin. Moreover, Ang-1 elicited the activation of the SAPK (stress-activated protein kinase)/JNK (c-Jun N-terminal kinase) MAPK, involved in axonogenesis. Our work shows a proneurogenic effect of Ang-1, highlighting the relevance of blood vessel/stem cell cross talk in health and disease. |
| Databáze: | OpenAIRE |
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