Fast analysis of sugars, fruit acids, and vitamin C in sea buckthorn (Hippophaë rhamnoides L.) varieties
| Autor: | Baoru Yang, Sigridur Jonsdottir, Heikki P. Kallio, Gudmundur G. Haraldsson, Katja Tiitinen |
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| Rok vydání: | 2006 |
| Předmět: |
Chromatography
Gas Magnetic Resonance Spectroscopy Carbohydrates Carboxylic Acids Malates Quinic Acid Berry Ascorbic Acid Fructose High-performance liquid chromatography chemistry.chemical_compound Botany Hippophae Food science Sugar Finland Vitamin C biology Chemistry Hippophae rhamnoides General Chemistry biology.organism_classification Ascorbic acid Glucose Fruit Dehydroascorbic acid Gas chromatography General Agricultural and Biological Sciences |
| Zdroj: | Journal of agricultural and food chemistry. 54(7) |
| ISSN: | 0021-8561 |
| Popis: | A fast, one-step gas chromatographic method was developed to analyze trimethylsilyl (TMS) derivatives of sugars, fruit acids, and ascorbic acid in sea buckthorn (Hippohae rhamnoides L.) berries. The method was applied to berry press juice of sea buckthorn of different origins grown in Finland during the 2003 and 2004 seasons. The method gave reliable results for d-fructose, d-glucose, ethyl-d-glucose, and malic, quinic, and ascorbic acids, which are the major sugars and acids in sea buckthorn juice. For the first time in sea buckthorn and evidently in any berry, the presence of ethyl β-d-glucopyranoside is reported. The structure of ethyl glucose was verified by high-performance liquid chromatography (HPLC), gas chromatography (GC), MS, and NMR analyses of both the isolated and the synthesized compounds. In the GC method, vitamin C was analyzed as ascorbic acid only, and dehydroascorbic acid was thus not taken into account. Keywords: Sea buckthorn (Hippophae rhamnoides L.); fruit acids; gas chromatography... |
| Databáze: | OpenAIRE |
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