Exogenous Classic Phytohormones Have Limited Regulatory Effects on Fructan and Primary Carbohydrate Metabolism in Perennial Ryegrass (Lolium perenne L.)
| Autor: | Anna eGasperl, Annette eMorvan-Bertrand, Marie-Pascale ePrud'Homme, Eric eVan Der Graaff, Thomas eRoitsch |
|---|---|
| Přispěvatelé: | Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), BMBF [1R15/2012, 27206ZE], Ministry of Education, Youth, and Sports of CR within the National Sustainability Program I (NPU I) [LO1415], Roitsch, Thomas |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sucrose fructan metabolism enzymatic activity Plant Science lcsh:Plant culture Biology Carbohydrate metabolism phytohormone 01 natural sciences Lolium perenne 03 medical and health sciences chemistry.chemical_compound Fructan Auxin fructan exohydrolase fructosyltransferase perennial ryegrass phytohormones primary carbohydrate metabolism Botany [SDV.BV]Life Sciences [q-bio]/Vegetal Biology lcsh:SB1-1110 Abscisic acid Gibberellic acid fructosyltransférase ComputingMilieux_MISCELLANEOUS Original Research 2. Zero hunger chemistry.chemical_classification Abiotic stress activité enzymatique métabolisme glucidique fructane exohydrolase food and beverages biology.organism_classification lolium pérenne 030104 developmental biology chemistry Biochemistry 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Frontiers, 2016, 6, ⟨10.3389/fpls.2015.01251⟩ Gasperl, A, Morvan-Bertrand, A, prud'homme, M-P, van der Graaff, E & Roitsch, T G 2016, ' Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass ( Lolium perenne L.) ', Frontiers in Plant Science, vol. 6, 1251 . https://doi.org/10.3389/fpls.2015.01251 Frontiers in Plant Science (6), . (2016) Frontiers in Plant Science, Vol 6 (2016) |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2015.01251⟩ |
| Popis: | International audience; Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs) and breakdown through fructan exohydrolases (FEHs). The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA), are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX), ethylene (ET), gibberellic acid (GA), or kinetin (KIN). The most pronounced fast effects were a transient increase of I- I activities by AUX, KIN, ABA, and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and sucrose pool. |
| Databáze: | OpenAIRE |
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