SMAUG is a major regulator of maternal mRNA destabilization in Drosophila and its translation is activated by the PAN GU kinase

Autor: Timothy P. Hughes, Wael Tadros, Terry L. Orr-Weaver, Fiona Menzies, Leah A. Vardy, Craig A. Smibert, Tomas Babak, Aaron L. Goldman, Howard D. Lipshitz, J. Timothy Westwood
Rok vydání: 2005
Předmět:
Untranslated region
Cytoplasm
Embryo
Nonmammalian
Polyadenylation
MRNA destabilization
RNA Stability
Regulator
Biology
Protein Serine-Threonine Kinases
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
stomatognathic system
Animals
Drosophila Proteins
Molecular Biology
3' Untranslated Regions
Heat-Shock Proteins
030304 developmental biology
Ovum
Regulation of gene expression
0303 health sciences
Models
Genetic
Gene Expression Profiling
Computational Biology
Gene Expression Regulation
Developmental
RNA-Binding Proteins
Translation (biology)
Cell Biology
Microarray Analysis
Molecular biology
Gene expression profiling
Repressor Proteins
RNA
Messenger
Stored
Drosophila melanogaster
Protein Biosynthesis
Mutation
Female
Smaug
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Developmental cell. 12(1)
ISSN: 1534-5807
Popis: SummaryIn animals, egg activation triggers a cascade of posttranscriptional events that act on maternally synthesized RNAs. We show that, in Drosophila, the PAN GU (PNG) kinase sits near the top of this cascade, triggering translation of SMAUG (SMG), a multifunctional posttranscriptional regulator conserved from yeast to humans. Although PNG is required for cytoplasmic polyadenylation of smg mRNA, it regulates translation via mechanisms that are independent of its effects on the poly(A) tail. Analyses of mutants suggest that PNG relieves translational repression by PUMILIO (PUM) and one or more additional factors, which act in parallel through the smg mRNA's 3′ untranslated region (UTR). Microarray-based gene expression profiling shows that SMG is a major regulator of maternal transcript destabilization. SMG-dependent mRNAs are enriched for gene ontology annotations for function in the cell cycle, suggesting a possible causal relationship between failure to eliminate these transcripts and the cell cycle defects in smg mutants.
Databáze: OpenAIRE
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