Clinical and genomic features of Corynebacterium macginleyi-associated infectious keratitis

Autor:	Holger Brüggemann, Birgitta Ejdervik Lindblad, Susanna Sagerfors, Bo Söderquist, Anja Poehlein, Mastaneh Afshar
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	0301 basic medicine Adult Male Science Population Single-nucleotide polymorphism Diseases Infectious Keratitis Biology Corynebacterium Genome Microbiology DNA sequencing Article 03 medical and health sciences 0302 clinical medicine Corynebacterium macginleyi Humans education Pathogen Aged Retrospective Studies Keratitis education.field_of_study Multidisciplinary Molecular medicine Corynebacterium Infections Whole Genome Sequencing Middle Aged Contact lens 030104 developmental biology 030221 ophthalmology & optometry Medicine Female
Zdroj:	Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) Scientific Reports Sagerfors, S, Poehlein, A, Afshar, M, Lindblad, B E, Brüggemann, H & Söderquist, B 2021, ' Clinical and genomic features of Corynebacterium macginleyi-associated infectious keratitis ', Scientific Reports, vol. 11, no. 1, pp. 6015 . https://doi.org/10.1038/s41598-021-85336-w
ISSN:	2045-2322
DOI:	10.1038/s41598-021-85336-w
Popis:	Infectious keratitis is a potentially sight threatening ophthalmological emergency. Contact lens wear is a common risk factor. Diagnostic advances such as MALDI-TOF MS provides new insights into the spectrum of corneal pathogens and on microbes previously considered as commensals. Corynebacterium macginleyi was described in 1995, and in 2018, the genomic features of three isolates were reported after whole-genome sequencing. Here we describe the clinical characteristics of patients with infectious keratitis (n = 29) presumably caused by Corynebacterium macginleyi, and analyze the genomic features of C. macginleyi (n = 22) isolated from the corneal ulcers of these patients. The disease course was uneventful apart from minor interventions such as corneal cross-linking and amniotic membrane transplant. Genome sequencing and comparison revealed a highly conserved core genome of C. macginleyi. Based on the analyses of single nucleotide polymorphisms, the population could be divided into two main clades that also differed in a few clade-specific genomic islands. Patients infected with an isolate belonging to the minor clade (n = 7) presented a more severe disease. Comparisons with other corynebacterial species clearly separated C. macginleyi. C. macginleyi may be considered a corneal pathogen; genomic analysis provided insights into its population structure and disease-causing potential.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f7bc152fe670636f18a64035f7ef47f https://doaj.org/article/b0e1d2813d824aa8983f3f09775001da Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.