Transcription factors expressed in soybean roots under drought stress
| Autor: | Norman Neumaier, Selma S. Pereira, A.A.P. Rolla, J. F. C. Carvalho, Maria Cristina Neves Oliveira, F. C. M. Guimaraes, Renata Stolf-Moreira, Alexandre Lima Nepomuceno, José Renato Bouças Farias |
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| Přispěvatelé: | Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Universidade Estadual Paulista (Unesp), Universidade Estadual de Londrina (UEL) |
| Rok vydání: | 2011 |
| Předmět: |
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Molecular Sequence Data Sequence alignment Biology Drought stress tolerance Genes Plant Plant Roots Gene Expression Regulation Plant Stress Physiological Gene expression Botany Genetics Gene family MYB Amino Acid Sequence Molecular Biology Transcription factor Gene Phylogeny Plant Proteins Regulation of gene expression Gene Expression Profiling fungi food and beverages General Medicine Droughts Gene expression profiling Soybeans Soybean Sequence Alignment Real-time PCR Transcription Factors |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1676-5680 |
| Popis: | Made available in DSpace on 2013-09-27T14:52:55Z (GMT). No. of bitstreams: 1 WOS000300617600142.pdf: 5173765 bytes, checksum: 2d98eab9efce2f7e70b5410a1e758230 (MD5) Previous issue date: 2011-01-01 Made available in DSpace on 2013-09-30T19:24:39Z (GMT). No. of bitstreams: 1 WOS000300617600142.pdf: 5173765 bytes, checksum: 2d98eab9efce2f7e70b5410a1e758230 (MD5) Previous issue date: 2011-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:34:43Z No. of bitstreams: 1 WOS000300617600142.pdf: 5173765 bytes, checksum: 2d98eab9efce2f7e70b5410a1e758230 (MD5) Made available in DSpace on 2014-05-20T15:34:43Z (GMT). No. of bitstreams: 1 WOS000300617600142.pdf: 5173765 bytes, checksum: 2d98eab9efce2f7e70b5410a1e758230 (MD5) Previous issue date: 2011-01-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) To gain insight into stress-responsive gene regulation in soybean plants, we identified consensus sequences that could categorize the transcription factors MYBJ7, BZIP50, C2H2, and NAC2 as members of the gene families myb, bzip, c2h2, and nac, respectively. We also investigated the evolutionary relationship of these transcription factors and analyzed their expression levels under drought stress. The NCBI software was used to find the predicted amino acid sequences of the transcription factors, and the Clustal X software was used to align soybean and other plant species sequences. Phylogenetic trees were built using the Mega 4.1 software by neighbor joining and the degree of confidence test by Bootstrap. Expression level studies were carried out using hydroponic culture; the experiments were designed in completely randomized blocks with three repetitions. The blocks consisted of two genotypes, MG/BR46 Conquista (drought-tolerant) and BR16 (drought-sensitive) and the treatments consisted of increasingly long dehydration periods (0, 25, 50, 75, and 100 min). The transcription factors presented domains and/or conserved regions that characterized them as belonging to the bzip, c2h2, myb, and nac families. Based on the phylogenetic trees, it was found that the myb, bzip and nac genes are closely related to myb78, bzip48 and nac2 of soybean and that c2h2 is closely related to c2h2 of Brassica napus. Expression of all genes was in general increased under drought stress in both genotypes. Major differences between genotypes were due to the lowering of the expression of the mybj7 and c2h2 genes in the drought-tolerant variety at some times. Over-expression or silencing of some of these genes has the potential to increase stress tolerance. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Soja, Londrina, PR, Brazil Univ Estadual São Paulo Julio de Mesquita Filho, Jaboticabal, SP, Brazil Universidade Estadual de Londrina (UEL), Londrina, PR, Brazil Univ Estadual São Paulo Julio de Mesquita Filho, Jaboticabal, SP, Brazil |
| Databáze: | OpenAIRE |
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