Enhancement by interleukin-1 (IL-1) of plasminogen activator inhibitor (PA-I) activity in cultured human endothelial cells
| Autor: | I. Millet, Maria Benedetta Donati, Elisabetta Dejana, M. Gramse, G. Pintucci, J. Van Damme, Luciana Mussoni, F. Breviario |
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| Rok vydání: | 1986 |
| Předmět: |
Urokinase
Time Factors Dose-Response Relationship Drug Chemistry Biophysics Sodium Dodecyl Sulfate Interleukin Cell Biology Biochemistry Molecular biology Molecular Weight Plasminogen Inactivators Antigen Pi medicine Humans Endothelium Beta (finance) Molecular Biology Plasminogen activator Cells Cultured Glycoproteins Interleukin-1 medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 139:720-727 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(86)80050-x |
| Popis: | Summary Cultured human endothelial cells produce increased levels of PA-I when stimulated with IL-1. The stimulatory effect was elicited by both alfa (pI 5) and beta (pI 7) IL-1 molecules. The effect was both dose and time-dependent, plateau reached after 8 h. The PA-I measured in cell extracts was SDS-resistant with an apparent m.w. of 53 Kd and inhibited both tissue-type plasminogen activator (t-PA) and urokinase (UK). No change in t-PA antigen level was detected with any of the IL-1 preparations tested. |
| Databáze: | OpenAIRE |
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