Constituents of Cryptotaenia japonica Inhibit Melanogenesis via CREB- and MAPK-Associated Signaling Pathways in Murine B16 Melanoma Cells
| Autor: | Sung-Yoon Lee, Zuh-Kyung Seong, Hyeong-Kyu Lee, Sei-Ryang Oh, Amrit Poudel |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Cryptotaenia japonica murine B16 melanoma cells flavonoids anti-melanogenic effect MEK hyperpigmentation Tyrosinase Pharmaceutical Science CREB Article Analytical Chemistry Melanin lcsh:QD241-441 03 medical and health sciences 0302 clinical medicine lcsh:Organic chemistry Drug Discovery Ultraviolet light Physical and Theoretical Chemistry biology integumentary system Organic Chemistry Microphthalmia-associated transcription factor Cell biology 030104 developmental biology Biochemistry Chemistry (miscellaneous) 030220 oncology & carcinogenesis biology.protein Molecular Medicine Phosphorylation Signal transduction |
| Zdroj: | Molecules Molecules; Volume 21; Issue 10; Pages: 1296 Molecules, Vol 21, Iss 10, p 1296 (2016) |
| ISSN: | 1420-3049 |
| Popis: | Melanin plays an important role in protecting the skin against ultraviolet light and is responsible for skin color. However, overproduction of melanin is related to several skin disorders, such as age spots, freckles, cafe au lait spots, Becker’s nevus and other hyperpigmentation syndromes. The aim of this study was to identify the effects of kaempferol-7-O-β-d-glucuronide (K7G) and tilianin, isolated from Cryptotaenia japonica, on melanogenesis and their mechanisms of action in murine B16 melanoma cells. The α-melanocyte-stimulating hormone (α-MSH)-induced melanin production was significantly inhibited by K7G and tilianin in a dose-dependent manner. The effects of these compounds on the signaling pathway of melanogenesis were examined. K7G and tilianin downregulated the expression of microphthalmia-associated transcription factor (MITF) and melanocyte-specific enzymes, i.e., tyrosinase and TRP1. These compounds also inhibited the phosphorylation of cyclic adenosine monophosphate (cAMP)-response element binding protein (CREB) in a dose-dependent manner. In addition, these compounds increased the phosphorylation of extracellular signal-regulated kinase (ERK) but decreased the phosphorylation of c-Jun N-terminal kinase (JNK) in B16 cells. Based on the above results, the anti-melanogenic effects of these compounds are caused by suppression of the MAPK signaling pathway through the down-regulation of α-MSH-induced CREB accumulation. This finding suggests that K7G and tilianin may be good candidates for further research to develop therapeutic agents for hyperpigmentation diseases. |
| Databáze: | OpenAIRE |
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