Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape
| Autor: | Yanye Ruan, Zhu Ys, Cui Zh, Wang P, Liu K, Fan Jj, Xu X, Zhang Lj |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Molecular Sequence Data
Genes Plant Real-Time Polymerase Chain Reaction chemistry.chemical_compound Rapid amplification of cDNA ends Gene Expression Regulation Plant Complementary DNA Botany Genetics Gene family Vitis Amino Acid Sequence Molecular Biology Abscisic acid Gene Disease Resistance Plant Diseases Plant Proteins Peronospora biology Abiotic stress General Medicine biology.organism_classification Protein Structure Tertiary Up-Regulation chemistry Plasmopara viticola Downy mildew |
| Zdroj: | Genetics and molecular research : GMR. 14(3) |
| ISSN: | 1676-5680 |
| Popis: | Grapevine downy mildew, caused by Plasmopara viticola, is a devastating disease that results in considerable economic losses as well as environmental damage through the repeated application of fungicides. The nucleotide-binding site leucine-rich repeat gene family functions in plant immunoactivity against various pathogens and pests. In this study, the 5' and 3' ends of the resistance gene homology fragment RGA5 were obtained by rapid amplification of cDNA ends. The 4282-base pair full-length cDNA was obtained using gene-specific primers, and the corresponding 1335-amino acid protein sequence contained characteristic nucleotide-binding site leucine-rich repeat domains of plant resistance proteins, including the toll-interleukin receptor type region. Expression of RGA5 during P. viticola infection and abiotic stress was investigated using quantitative real-time polymerase chain reaction. The results showed that treatment with P. viticola and 4 abiotic stimuli (salicyclic acid, methyl-jasmonate, abscisic acid, H2O2) significantly induced RGA5 within 12 days of inoculation. Therefore, RGA5 may play a critical role in protecting grapevines against P. viticola via signaling pathways involving these molecules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|