Investigation of dynamic thiol–disulphide homoeostasis in age-related cataract patients with a novel and automated assay
| Autor: | Fatma Uçar, Havva Kocak, Fatih Özcura, Hacı Murat Sağdık, Mehmet Tetikoğlu, Serdar Aktas, Funda Eren, Salim Neselioglu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Disulphide Thiobarbituric acid Oxidative phosphorylation medicine.disease_cause Cataract 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Malondialdehyde medicine Homeostasis Humans Disulfides Sulfhydryl Compounds Aged chemistry.chemical_classification biology business.industry Middle Aged Catalase Ophthalmology 030104 developmental biology Endocrinology Biochemistry chemistry Oxidative stress Case-Control Studies 030221 ophthalmology & optometry Thiol biology.protein Female business Age-related cataract |
| Popis: | WOS: 000431304900026 PubMed: 28397146 Purpose The aim of this study was to determine plasma thiol-disulphide homoeostasis in patients with age-related cataract (ARC) and compare the results of the patients with healthy subjects. Plasma malondialdehyde (MDA) levels and catalase (CAT) activity were also investigated. Methods The study included 53 cataract patients and 52 healthy volunteers. Native thiol-disulphide exchanges were determined using a novel and automated method. CAT activity was determined using the method described by Aebi, and MDA levels were calculated using the thiobarbituric acid method. Result Native thiol and total thiol levels were significantly lower in the cataract patients compared with the controls (p < 0.001, p = 0.002, respectively). The disulphide levels of the cataract patients were significantly higher than the controls (p = 0.002). The ratios of disulphide/native thiol and disulphide/ total thiol were statistically higher in the cataract patients compared with the control group (p < 0.001, p < 0.001, respectively). Furthermore, CAT activity was significantly lower in the cataract patient group compared with the control group (p < 0.001), and MDA levels were insignificantly higher in the patient group (p = 0.581). Conclusions Our study showed that dynamic thioldisulphide homoeostasis has shifted towards disulphide formation, as a result of thiol oxidation in ARC patients. The present study is the first to measure thiol-disulphide homoeostasis in ARC patients with a novel automated assay. This study supports the hypothesis that cataract is an oxidative disorder. Further studies are required in order to examine the relationship between oxidative stress and the development of cataract formation. |
| Databáze: | OpenAIRE |
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