Leaves of Persimmon (Diospyros kaki Thunb.) Ameliorate N-Methyl-N-nitrosourea (MNU)-Induced Retinal Degeneration in Mice
| Autor: | Sung Jae Yang, Hong Ryul Ahn, Suk Woo Kang, Kyung-A Kim, Sang Hoon Jung, Youngwoo Song |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Retinal degeneration Nerve Tissue Proteins Biology Pharmacology Nestin Mice chemistry.chemical_compound Glial Fibrillary Acidic Protein Retinitis pigmentosa medicine Animals Humans Outer nuclear layer Glial fibrillary acidic protein Plant Extracts Retinal Degeneration Diospyros kaki Methylnitrosourea Retinal General Chemistry Anatomy Diospyros medicine.disease Mice Inbred C57BL Plant Leaves Disease Models Animal medicine.anatomical_structure chemistry biology.protein sense organs General Agricultural and Biological Sciences Quercetin |
| Zdroj: | Journal of Agricultural and Food Chemistry. 63:7750-7759 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.5b02578 |
| Popis: | The purpose of the study was to investigate the protective effects of the ethanol extract of Diospyros kaki (EEDK) persimmon leaves to study N-methyl-N-nitrosourea (MNU)-induced retinal degeneration in mice. EEDK was orally administered after MNU injection. Retinal layer thicknesses were significantly increased in the EEDK-treated group compared with the MNU-treated group. The outer nuclear layer was preserved in the retinas of EEDK-treated mice. Moreover, EEDK treatment reduced the MNU-dependent up-regulation of glial fibrillary acidic protein (GFAP) and nestin expression in Müller and astrocyte cells. EEDK treatment also inhibited MNU-dependent down-regulation of rhodopsin expression. Quercetin exposure significantly attenuated the negative effects of H2O2 in R28 cells, suggesting that quercetin can act in an antioxidative capacity. Thus, EEDK may be considered as an agent for treating or preventing degenerative retinal diseases, such as retinitis pigmentosa and age-related macular degeneration. |
| Databáze: | OpenAIRE |
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